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Composition to enhance HDL cholesterol and to decrease intima-media thickening in animals and humans and a method for its preparation

Inactive Publication Date: 2005-12-22
ARJUNA NATURAL EXTRACTS LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0027] The objective of the present invention is to provide a safe natural product for treatment of lipid disorders connected with coronary heart disease and stroke without the disadvantages associated with the use of synthetic lipid lowering agents such as statins. More specifically, the invention aims at providing a composition which lowers the harmful LDL cholesterol and at the same time increasing the beneficial HDL cholesterol contents. Low HDL cholesterol is considered as the second most important predictor of coronary heart disease (CHD).
[0028] A second objective of the invention is to provide for a composition for potential reduction of intima-media thickening observed in heart patients and which is considered as an important marker and predictor of CHD.
[0029] A further objective of the present invention is to provide an easy and economical process for the commercial preparation of E. officinalis extract offering the benefits described above.

Problems solved by technology

In humans, infusing either of these complexes or pro-apo-A-I results in short term increase in HDL cholesterol, biliary cholesterol and fecal cholesterol loss, reinforcing the concept that elevating the HDL cholesterol level decreases the risk of cardiovascular disease.
Statins also reduce the serum TG levels; but they are often insufficient.
Statins are ineffective in the treatment of patients with chylomicronemia.
Thus, diets alone or in conjunction with lipid lowering drugs fail to yield the desired goal of safe lipid lowering.
Absolute IMT was also related to risk for clinical coronary events.
The extraction process described by Ghosal involves treating the fruit pulp with water containing 1% sodium chloride which was then left at room temperature for 12 hours followed by keeping the mixture at 10° C. for 3 days and thus is very time-consuming, costly and tedious.
This may not be desirable, given the adverse affects of salts for patients with hypertension which is closely associated with cardiac diseases.

Method used

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  • Composition to enhance HDL cholesterol and to decrease intima-media thickening in animals and humans and a method for its preparation
  • Composition to enhance HDL cholesterol and to decrease intima-media thickening in animals and humans and a method for its preparation

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0044] Five hundred kilograms of fresh amla fruits were pulped with an equal quantity of demineralized water and the slurry was treated with 2 wt % of pectinase enzyme under stirring at room temperature for 6 h and then filtered to yield 310 litres of the extract with a solids content of 4.8%. This solution was then concentrated below 60° C. to obtain a slurry with a solids content of 15.2%. This was then spray-dried (inlet temperature 180° C., outlet temperature 90° C.) to obtain 13.5 kg of a free flowing powder. The emblicanin A content of this preparation was 10.2%.

example 2

Toxicity Studies

Acute Toxicity

[0045] Healthy albino mice of either sex, having body weight 20-25 g were used. They were housed in clean polypropylene cages with food and water available ad libitum. After acclimatization for one week, their body weights were recorded and were divided into 8 groups of 6 each. Group A served as control and the remaining 7 groups were kept as experimental group. The experimental animals were supplied 200 mg, 400 mg, 600 mg, 800 mg, 2.5 g, 5 g and 10 g / kg of amla extract, respectively, orally after an overnight fasting. Animals were observed continuously for the first 6 h and mortality was recorded for 72 hours.

[0046] Amla extract up to a dosage level of 10 g / kg body weight produced no adverse effects such as increased motor activity, tremors, clonic convulsions, piloerection, muscle spasm, hyperesthesia, ataxia, sedation, hypnosis and analgesia. No mortality was recorded in 72 hours.

Sub Acute Toxicity

[0047] Thirty healthy male Sprague-Dawly rats ...

example 3

[0057] Male NZ white rabbits weighing 1.3-1.6 kg were individually caged and fed a normal standard diet. After an acclimatization period, they were divided into 4 groups of 5 animals each. One group (Group A) served as control and groups B 1, B2 and B3 served as experimental groups. The experimental groups were made hypercholesterolemic by feeding 100 mg cholesterol along with the diet daily for 4 months. After 4 months, Group B 1 was kept as untreated hypercholesterolemic control and the remaining two groups (B2 and B3) were fed orally with amla extract in the dosage of 10 mg and 20 mg / kg / day, respectively, for additional 4 months. Body weights of animals were recorded every 15 days.

[0058] Before starting the experiment, fasting blood was collected from all animals for estimation of serum total cholesterol, LDL cholesterol (LDL-C), HDL cholesterol (HDL-C) and triglycerides (TG). Blood samples were also analyzed for haematological parameters (RBC, WBC, haemoglobin (Hb) and lymphocy...

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PUM

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Abstract

A method of producing a product to correct hypercholesterolemia comprising pulping fruits of Emblica Officinalis with demineralized water to create a slurry. The slurry is treated with pectinase. The slurry is filtered to create a solution. The solution is concentrated to create a product.

Description

TECHNICAL FIELD [0001] This is a Continuation of International Application PCT / IN2003 / 000137, with an international filing date of Apr. 3, 2003 and claiming priority of India Patent Application 169 / MAS / 2003 filed on Mar. 3, 2003, both of which documents are incorporated herein by reference.[0002] This invention relates to a product (nutritional supplement) to correct dyslipidemia in animals and humans and to combat intima-media thickening thus affording significantly improved therapeutic and prophylactic cardioprotection, the said composition being an extract of Emblica officinalis, Gaertn (Euphorbiaceae) prepared without using any organic solvent and without resorting to any chemical processing steps. BACKGROUND OF THE INVENTION [0003]E. officinalis (Amla) fruit is one of the key constituents of the celebrated Ayurvedic preparation, Chyavanaprash, used in India for thousands of years as a vitalizing and rejuvenating health tonic. The low molecular weight hydrolyzable, gallo- and el...

Claims

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Application Information

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IPC IPC(8): A61K36/00A61K36/185
CPCA61K36/185A61K36/47A61K36/78A61P3/00A61P3/06A61P9/10
Inventor ANTONY, BENNY
Owner ARJUNA NATURAL EXTRACTS LTD