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Novel TNF receptor death domain ligand proteins and inhibitors of ligand binding

a technology of tnf receptor and ligand protein, which is applied in the direction of peptide/protein ingredients, dna/rna fragmentation, fungi, etc., can solve the problems that the ligands which bind to the tnf-r intracellular domain have yet to be identified, so as to prevent or ameliorate an inflammatory condition

Inactive Publication Date: 2009-04-09
LIN LIH LING +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ligands which bind to the TNF-R intracellular domain have yet to be identified.

Method used

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  • Novel TNF receptor death domain ligand proteins and inhibitors of ligand binding
  • Novel TNF receptor death domain ligand proteins and inhibitors of ligand binding
  • Novel TNF receptor death domain ligand proteins and inhibitors of ligand binding

Examples

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example 1

Cloning of TNF-R Death Domain Ligand Protein Encoding Polynucleotide

[0183]A yeast genetic selection method, the “interaction trap” [Gyuris et al. Cell 75:791-803, 1993, which is incorporated herein by reference], was used to screen W138 cell cDNA libraries (preparation, see below) for proteins that interact with the death domain of the P55 type 1 TNF receptor (TNF-R1-DD). A polynucleotide encoding amino acids 326 to 413 of the P55 type TNF receptor, TNF-R1-DD, was obtained via the polymerase chain reaction (PCR) using a grafting method. This TNF-R1-DD DNA was then cloned into pEG202 by BamHI and SalI sites, generating the bait plasmid, pEG202-TNF-R1-DD. This plasmid contains the HIS3 selectable marker, and expression of the bait, the LexA-TNF-R1-DD fusion protein, is from the strong constitutive ADH1 promoter. To create the reporter strain carrying the bait protein, yeast strain EGY48, containing the reporter sequence LexAop-Leu2 in place of the chromosomal LEU2, was transformed wit...

example 2

Expression of the TNF-R1-DD Ligand Protein

[0191]cDNAs encoding TNF-R intracellular ligand proteins were released from the pJG4-5 vector with the appropriate restriction enzymes. For example, EcoRI and XhoI or NotI and XhoI were used to release cDNA from clone 2DD and clone 20DD. Where the restriction sites were also present in the internal sequence of the cDNA, PCR was performed to obtain the cDNA. For example, the cDNA fragment encoding “clone 3DD” was obtained through PCR due to the presence of an internal XhoI site. These cDNAs were then cloned into various expression vectors. These included pGEX (Pharmacia) or pMAL (New England Biolabs) for expression as a GST (Glutathione-S-transferase) or MBP (maltose binding protein) fusion protein in E. coli, a pED-based vector for mammalian expression, and pVL or pBlueBacHis (Invitrogen) for baculovirus / insect expression. For the immunodetection of TNF-R intracellular ligand expression in mammalian cells, an epitope sequence, “Flag,” was in...

example 3

Assays of TNF-R Death Domain Binding

[0193]Two different methods were used to assay for TNF-R1-DD ligand protein activity. The first assay measures binding in the yeast strain in “interaction trap,” the system used here to screen for TNF-R1-DD interacting proteins. In this system, the expression of reporter genes from both LexAop-Leu2 and LexAop-LacZ relies on the interaction between the bait protein, in this case TNF-R1DD, and the prey, the TNF-R intracellular ligand. Thus, one can measure the strength of the interaction by the level of Leu2 or LacZ expression. The most simple method is to measure the activity of the LacZ encoded protein, β-galactosidase. This activity can be judged by the degree of blueness on the X-Gal containing medium or filter. For the quantitative measurement of β-galactosidase activity, standard assays can be found in “Methods in Yeast Genetics” Cold Spring Harbor, N.Y., 1990 (by Rose, M.D., Winston, F., and Hieter, P.).

[0194]The second assay for measuring bi...

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Abstract

Novel TNF receptor death domain (“TNF-R1-DD”) ligand proteins are disclosed. Polynucleotides encoding the TNF-R1-DD ligand protein are also disclosed, along with vectors, host cells, and methods of making the TNF-R1-DD ligand protein. Pharmaceutical compositions containing the TNF-R1-DD ligand protein, methods of treating inflammatory conditions, and methods of inhibiting TNF-R death domain binding are also disclosed. Methods of identifying inhibitors of TNF-R death domain binding and inhibitors identified by such methods are also disclosed.

Description

[0001]This application is a continuation-in-part of application Ser. No. 08 / 602,228, filed Feb. 15, 1996, which was a continuation-in-part of application Ser. No. 08 / 533,901, filed Sep. 26, 1995, which was a continuation-in-part of application Ser. No. 08 / 494,440, filed Jun. 19, 1995, which was a continuation-in-part of application Ser. No. 08 / 327,514, filed Oct. 19, 1994.BACKGROUND OF THE INVENTION[0002]The present invention relates to the field of anti-inflammatory substances and other substances which act by inhibiting binding to the intracellular domain of a tumor necrosis factor receptor (hereinafter “TNF-R”), such as, for example, the P55 type (or TNF-R1) TNF receptor. More particularly, the present invention is directed to novel ligands which bind to the TNF-R intracellular domain and to inhibition or modulation of signal transduction by this receptor.[0003]Tumor necrosis factor (herein “TNF”) is a cytokine which produces a wide range of cellular activities. TNF causes an inf...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/395C12N15/11C12N5/06C12P21/04C07K14/00C12Q1/68A61K38/16C07K16/18G01N33/566A61K38/00C12N15/09A61K45/00A61K48/00A61P29/00C07K14/47C07K14/715C07K16/24C12N1/19C12N5/10C12N15/12C12P21/02
CPCA61K38/00C07K14/47C07K2317/34C07K16/24C07K14/4743A61P29/00
Inventor LIN, LIH-LINGCHEN, JENNIFERSCHIEVELLA, ANDREA R.GRAHAM, JAMES
Owner LIN LIH LING
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