Process for the production of a hybridoma and antibody obtained therefrom, able to recognize more than one vitamin d metabolite

Inactive Publication Date: 2011-04-28
DIASOURCE IMMUNOASSAYS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0092]The fact that the monoclonal antibody obtained is able to identify 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 is extremely surprising and overcomes a technical obstacle whereby such property could not be obtained using a monoclonal antibody originating from a single hybridoma. A significant advantage of the process of the invention i

Problems solved by technology

However, measuring the content of vitamin D is actually of little value, since vitamin D concentrations fluctuate significantly based on the oral consumption of vitamin D2.
However, most frequently, this leads to the production of a mixture of antibodies, this mixture randomly binding more than one antigen, thus leading to unspecific measurement.
A first issue is the life span of the host animal, Frequently, multiple animals needed to be immunised because of biological variability, Some animals being the source of the most specific antisera have to be selected and the death of said animals lead to the end of the production of the desired antibodies, until next immunisation of animals.
Secondly, the antiserum reactivity is also a concern because the desired antibo

Method used

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  • Process for the production of a hybridoma and antibody obtained therefrom, able to recognize more than one vitamin d metabolite
  • Process for the production of a hybridoma and antibody obtained therefrom, able to recognize more than one vitamin d metabolite
  • Process for the production of a hybridoma and antibody obtained therefrom, able to recognize more than one vitamin d metabolite

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[0093]The present invention shall be described according to a specific form of execution in which the hapten, used for the immunisation of the animal, is a derivative of formula (I) in which n is an integer equal to 0; R, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11, R14, R15 are hydrogen; R12 and R13 are a methyl group. This hapten shall be referred to as (H) for the remaining description. It is important to understand that the invention is not limited to this embodiment. For example, the hapten may be a derivative of formula (I) in which n is equal to 1, 2 or 3 with substituents unchanged.

Procedure for the Production of a Monoclonal Antibody

[0094]The production of a monoclonal antibody may be effected following the conventional procedure such as described, for example, in Kohler and Milstein, Nature 1975 (256), 495-497 or Eur. J. Immunol. 1976 (6), 511-519. According to this method, myeloid cells are combined with lymphocytic B cells of an immunised animal to obtain hybrid cells, know...

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Abstract

The invention concerns a process for the production of a hybridoma, and of a monoclonal antibody or fragments thereof able to recognize 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This application claims the benefit of U.S. Provisional Patent Application No. 61 / 255,164, filed Oct. 27, 2009, of PCT Patent Application No. PCT / EP2009 / 064148, filed Oct. 27, 2009, and Belgian Provisional Patent Application No. BE 2010 / 0234, filed Apr. 12, 2010, the entirety of each which are hereby incorporated by reference into this application.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The invention concerns a process for the production of a hybridoma and a monoclonal antibody obtained therefrom, able to recognize one epitope present on more than one antigen. In particular, the invention relates to a process for the production of a hybridoma and a monoclonal antibody, or fragments thereof, able to recognize more than one vitamin D metabolites, namely 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3.[0004]2. Description of Related Art[0005]Vitamin D is the generic term used to designate vitamin D2 or ergocalciferol and...

Claims

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Application Information

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IPC IPC(8): G01N33/53C12P21/08C12N5/16C07K16/18C12N15/06C12N15/07C12M1/34G01N33/50G01N30/00
CPCC07K16/26C07K16/44Y10T436/203332G01N33/82C07K2317/33C07K2317/24G01N33/577
Inventor ANCIAUX, MICHELMATHIEU, FABIENNELIN, FREDERICPONCELET, MARTIN
Owner DIASOURCE IMMUNOASSAYS
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