Non-caloric probiotic composition and method of preparation
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example 1
Procedure for Adding the Probiotic Microbial Strain to Drinking Water
[0058]The procedure for adding the probiotic to the water is as follows. The process is done as a batching process. Filtered or other drinking water is batched into tanks that preferably have the capability to mix at a moderate speed (e.g. about 1500 rpm). The probiotic microbial strain, preferably in the form of spores, is added using a geometric addition system with two steps of 10 fold dilution. If 500 g were to be added in total to a 1,000-gallon batch, the 500 g would first be added and mixed into a 10-gallon pre-mix batch. Then, the 10-gallon pre-mix batch would be added to a 100-gallon secondary mix batch and mixed for 5-7 minutes. The 100-gallon secondary mix would finally be added to the 1,000-gallon batch tank and mixed for 5-7 minutes.
example 2
Stability of Bacillus Spores in Formulated Drinking Water
[0059]Bacillus spores were combined with a mixture of carriers consisting of a combination of Gum Acacia, ascorbyl palmitate, TWEEN® 80 (polyethylene glycol sorbitan monooleate), and Span® 40 (sorbitan monopalmitate) and then added to filtered drinking water using the procedure outlined in Example 1. The stability and viability of the Bacillus spores was tested over a 6-month period.
[0060]The conditions for storage of the product were room temperature with high relative humidity. The initial inoculum level was maintained within less than a 1 log variation and typically within the minimum detection level for the plating method (see FIG. 1). This study demonstrated the effectiveness of the formulation at preserving the spores in drinking water without the need of flavoring agents and without causing any odor in the drinking water.
example 3
Serial Dilution of Bacillus atrophaeus (Bacillus subtilis) Spores in Probiotic Drink
Methodology
[0061]Bacillus subtilis HU58 powder 1×1011 (a spores suspension—American Type Culture Collection #9372) was diluted in spring water to a concentration of approximately 4×106 as described in greater detail in the steps below. The addition of the probiotic spores to the water was done in an increasing serial concentration method to ensure proper distribution and suspension of the strains in the final volume of desired concentration.
[0062]Serial concentration to achieve a desired concentration of one embodiment of a probiotic drink including the following steps.[0063]1. 160 mg of powder (1.6×1010) was added to 40 mL (approximately 0.0105 gal) of spring water and mixed for 5 to 7 minutes. The resulting concentration was expected to be approximately 4×108 CFU / mL.[0064]2. The entire contents of the solution from step (1.) was added to 360 mL (approx. 0.105) gal) of spring water and mixed for 5 t...
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Abstract
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