Compositions on plasma-treated surfaces

a technology of plasma-treated surfaces and compositions, applied in the field of compositions on plasma-treated surfaces, can solve the problems of insufficient adhesion, compositions may become dislocated or loose, dislocated or loose compositions may complicate or prevent reconstitution, etc., to improve the hydrophilicity of the surface and therefore its wettability, prevent or reduce the frequency of dislocation or loosening of solid compositions, and improve the effect of adhesion

Active Publication Date: 2018-04-05
GEN PROBE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0003]Compositions, e.g., comprising bulking reagents or reagents for performing chemical or biochemical reactions or assays, are provided as solid compositions adhered to a plasma-treated surfaces. It has been found that plasma treatment of a surface can improve the adhesion to the surface of solid compositions formed by drying a liquid solution, and that plasma treatment can prevent or reduce the frequency of dislocation or loosening of solid compositions from a surface, such as the surface at or near the bottom of a vessel. Without wishing to be bound to a particular theory, plasma treatment may increase the hydrophilicity of the surface and therefore its wettability, strengthening the interaction of the reagent with the surface, and that the strengthened interaction persists following a drying process, e.g., lyophilization.
[0004]The present disclosure is premised in part on the insight that the adhesion of a solid composition such as a lyophilized pellet to a surface such as a plastic can be strengthened by plasma treatment of the surface before the solid composition is in contact with the surface. Accordingly, the compositions, methods, and kits disclosed herein can provide products with improved ability to survive agitation that may occur during processes such as shipping and handling, or at least provide the public with a useful choice.

Problems solved by technology

Lack of sufficient adhesion can be a problem in areas such as providing solid pharmaceutical compositions or reagents for performing chemical, pharmaceutical, or biochemical reactions or assays, in that the composition may become dislocated or loose during shipment or handling.
Dislocated or loose compositions may complicate or prevent reconstitution, such as where a fixed volume of reconstitution liquid is dispensed in an instrument and some or all of the composition is no longer adhered at or near the bottom of the vessel in which reconstitution occurs.
Failure to completely reconstitute the composition into a liquid state can adversely affect assay performance.

Method used

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  • Compositions on plasma-treated surfaces
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  • Compositions on plasma-treated surfaces

Examples

Experimental program
Comparison scheme
Effect test

example 1

eatment with Short-Cycle Lyophilization

[0164]FIGS. 2A-2B show bar graphs in which the height of the each bar indicates the number of drops withstood before a pellet became loose in twelve wells each of an untreated cartridge (“None”), or four plasma-treated cartridges (“Plasma1” through “Plasma4”). Lyophilization was performed using a shorter cycle.

[0165]The adhesion of pellets to the wells was noticeably stronger in the plasma-treated wells. In the untreated wells, the pellets became loose following an average of 9.1 drops, while for the Plasma1, Plasma2, Plasma3, and Plasma4 treated wells, the pellets became loose following an average of 46.6, 62.2, 55.7, and 59.4 drops. respectively.

[0166]Additional experiments were performed using cartridges treated with plasma in air and O2. In these experiments, the pellets became loose following an average number of drops between 74 and 88 (data not shown).

example 2

eatment with Long-Cycle Lyophilization

[0167]FIGS. 3A-3B show bar graphs in which the height of the each bar indicates the number of drops withstood before a pellet became loose in twelve wells each of an untreated cartridge (“None”), two cartridges treated with plasma in air (“Plasma / Air 1” and “Plasma / Air 2”), and two cartridges treated with plasma in O2 (“Plasma / O2 1” and “Plasma / O2 2”). Lyophilization was performed using a longer cycle.

[0168]The adhesion of pellets to the wells was noticeably stronger in the plasma-treated wells. In the untreated wells, the pellets became loose following an average of 12.5 drops, while for the Plasma / Air 1, Plasma / Air 2, Plasma / O2 1, Plasma / O2 2 treated wells, the pellets became loose following an average of 36.9, 48.2, 38.8, and 45.0 drops, respectively.

[0169]Cartridges subjected to the Plasma1, Plasma2, Plasma3, and Plasma4 treatments as in Example 1 were filled with trehalose solution and lyophilized using a longer cycle. The pellets became lo...

example 3

eated Cartridge Storage

[0170]Cartridges were subjected to plasma treatment as in Example 1. Following plasma treatment, the cartridges were stored in an oxygen containing environment for a period of time of either 1-day, 30-days, 90-days or 100-days. At the end of the storage period, wells in the cartridge were filled with a saccharide containing PCR reaction solution and the solution was lyophilized. Control cartridges that were not plasma-treated were also filled with the saccharide solution, lyophilized and stored for each of 1-day, 30-days, 90-days, and 100-days.

[0171]Following lyophilization, all of the cartridges containing lyophilized substances were subjected to package performance testing, which is designed to emulate shipping and handling conditions. The individual tests performed were (i) Initial Manual Handling, ASTM D5276-98 Standard, Schedule A, (ii) Stacked Vibration, ASTM D4728-06 Standard, Schedule D, (iii) Loose Load Vibration, ASTM D999-08 Standard, Schedule F, (i...

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Abstract

The disclosure provides solid compositions such as lyophilisates adhered to surfaces such as plasma-treated surfaces and related methods, uses, kits, intermediates, starting materials, and downstream products.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims benefit of priority under 35 U.S.C § 119(e) to provisional application No. 62 / 402,446, filed Sep. 30, 2016; the entire contents of which are incorporated herein by referenceINTRODUCTION AND SUMMARY[0002]Solid compositions do not necessarily adhere strongly to surfaces. Lack of sufficient adhesion can be a problem in areas such as providing solid pharmaceutical compositions or reagents for performing chemical, pharmaceutical, or biochemical reactions or assays, in that the composition may become dislocated or loose during shipment or handling. Dislocated or loose compositions may complicate or prevent reconstitution, such as where a fixed volume of reconstitution liquid is dispensed in an instrument and some or all of the composition is no longer adhered at or near the bottom of the vessel in which reconstitution occurs. Failure to completely reconstitute the composition into a liquid state can adversely affect assa...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68B01L3/00C08J7/12C08J7/043C08J7/048
CPCC12Q1/686B01L3/5085C08J7/123B01L2300/161B01L2200/12C08J2345/00B01L2200/16C08J7/065C08J7/18C08J7/048C08J7/043G01N3/303
Inventor KNIGHT, BYRON J.OPALSKY, DAVID
Owner GEN PROBE INC
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