Method and system for sensing
a sensing and method technology, applied in the field of sensing, can solve the problems of inability to direct test biosamples such as blood or serum, severe tissue dysfunction, and need preprocessing
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 1
System Fabrication
[0479]An exemplary microfluidic biosensing system (also referred to herein as a microfluidic array or chip), according to some embodiments of the present invention, which may be utilized as a nanowire biosensor for multiplex real-time monitoring of metabolites, is presented in FIGS. 1A and 1B.
[0480]The exemplary system included a culture compartment with several wells containing one or more solutions (e.g., solutions containing cells, reductant, and / or oxidase enzymes) arranged, individually or in combination, in the wells. The wells were in fluid communication via microchannels with a sensing compartment. The sensing compartment included a plurality of functionalized nanostructures. The solutions were introducible from the wells to the sensing compartment, by means of solenoid valves operative to close or to open fluid communication channels.
[0481]The valves allowed different samples to be switched for multiplex sensing.
[0482]In the sensing compartment, a SiNW FET...
example 2
Sensing
[0504]General Sensing Setup:
[0505]A data acquisition system was used to measure the current of a SiNW FET (Ids) induced by surface charges from ROS or H2O2, during oxidation of an analyte in an analyte solution, or from a reductant, during reduction of an analyte in an analyte solution.
[0506]For measurements of cellular metabolites / activity, cells were cultured in the chip, namely, in the culture compartment thereof (see, for example, FIG. 1A), while placing the chip in an incubator during measurements. A sample was introduced to the sensing compartment at 20 μl min−1 by using a syringe pump.
[0507]Voltage applied to the drain and source (Vds) was 0.2 V, while voltage to the gate (Vg) was determined from Ids-Vg characteristics before sensing.
[0508]Current-versus-time signals were recorded at 1-second intervals.
[0509]All acquired signals were reversed due to presetting of the data acquisition system. During a measurement, switching of samples may have intro...
example 3
Monitoring Metabolic Activity of Cells
[0544]Cell Culture, Drug Treatment and Viability Assessment:
[0545]Human T lymphocytes, Jurkat (TIB-152, ATCC), were cultured and incubated at 37° C. under a humidified 5% CO2 atmosphere. The culture medium used was RPMI 1640 medium (52400, GIBCO) with 10% fetal bovine serum (FBS; 04-001-1A, Biological Industry) and 1% penicillin / streptomycin (15140, GIBCO).
[0546]During experiments, cells were re-suspended in phenol red-free medium (11835-063, GIBCO) in the presence or absence of a drug, either methotrexate hydrate (MTX; M8407, SIGMA) or 2-deoxy-D-glucose (2DG; D6134, SIGMA), at a density of 1×106 cells / ml.
[0547]Cellular samples were dispensed into the wells of the sensing chip (see FIGS. 1A and 1B) in an incubator.
[0548]Cell viability was estimated by using a hemocytometer to count trypan blue-stained cells.
[0549]Isolation of Primary Human B Cells:
[0550]Peripheral blood (PB) cells were obtained from healthy donors and from p...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More