Peripherally-restricted dual-acting kappa and delta opioid agonist for analgesia in pain states involving the inflammatory response
a dual-acting, opioid agonist technology, applied in the direction of nervous disorders, drug compositions, organic chemistry, etc., can solve the problems of prescription opioids being highly addictive, unwanted side effects, and being widely misused, so as to enhance the kappa effect and reduce urinary output
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example 1
Model of Pain in Mice
[0120]The objective of this study was to evaluate the effects of Compound 1 and a peripherally-restricted kappa agonist (IC1204448) on formalin-evoked spontaneous nociceptive behaviors in mice. The study was performed by video recording of formalin-induced nociceptive behavior and then off-line scoring using a computer.
[0121]Subcutaneous plantar injection of formalin causes a bi-phasic nocifensive behavioral response in rodents. The early phase (phase 1) lasts for about 5-10 minutes, following which an interphase occurs without any discernible nociceptive reactions, after which the late phase (phase 2) nociceptive reaction ensues continuing from about 20-60 min following formalin injection. Thus, phase 2 of the formalin model is a model of continuously present, persistent pain, and is widely used for rapid screening of novel analgesic compounds. The model encompasses inflammatory, neurogenic and central mechanisms of nociception, and the late phase (phase 2), in...
example 2
Model of Pain in Rats
[0131]This study evaluated the efficacy of a single intraperitoneal injection of Compound 1 on hyperalgesic nociceptive behaviors in an CFA (Complete Freund's Adjuvant) Model of Rheumatoid Arthritis Pain in Rats.
[0132]Rats have been used as a reliable animal model for the study of pain due to many similarities of the peripheral and central nervous systems of rats and humans. These similarities are evident both in terms of behavioral responses to painful conditions and in terms of pain relieving effects of various therapeutic agents (i.e. opiates and nonsteroidal anti-inflammatory drugs) in both species.
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Animal Selection
[0133]A statistical power calculator (Massachusetts General Hospital on-line power calculator, http: / / hedwig.mgh.harvard.edu / sample_size / size.html) was used to determine the appropriate group size to ensure interpretable and reproducible results. Data from previous studies were input into the calculator and the group size was calculated base...
example 3
ic Pain Model in Rats
[0145]This study evaluated the efficacy of a single intraperitoneal injection of Compound 1 and the comparator, gabapentin, in the spinal nerve ligation (SNL) model for neuropathic pain in the rat. Rats have been used as a reliable animal model for the study of pain due to many similarities of the peripheral and central nervous systems of rats and humans. These similarities are evident both in terms of behavioral responses to painful conditions and in terms of pain relieving effects of various therapeutic agents (i.e. opiates and nonsteroidal anti-inflammatory drugs) in both species. Further, rats are vertebrates, which is necessary when investigating the effects of neuropathic pain.
Methods
Animal Selection
[0146]A statistical power calculator (Massachusetts General Hospital on-line power calculator, http: / / hedwig.mgh.harvard.edu / sample_size / size.html) was used to determine the appropriate group size to ensure interpretable and reproducible results. Data from prev...
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