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Detectably labeled porphyrin compound for identifying the sentinel lymph node

a technology of porphyrin compound and sentinel lymph node, which is applied in the field of detection labeled porphyrin compound for identifying can solve the problems of inability to radio-labeled, two separate injections, and inability to accurately identify the sentinel lymph nod

Inactive Publication Date: 2007-04-03
THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0016]The advantage of the present invention is that it combines in one compound and one injection the procedure that previously required two different compounds and two separate injections. The present invention in one embodiment therefore comprises a single purified porphyrin, rather than a mixture of porphyrins as in the known art. And, the present invention uses sodium salt instead of the ammonium salt of the known art.
[0017]The present invention in another embodiment comprises a non-radioactive metal (manganese in a preferred embodiment) attached to the porphyrin compound for identification of the sentinel lymph node using a Magnetic Resonance Imaging (MRI) examination.
[0018]In summary, the present invention in one embodiment provides a detectably labeled porphyrin compound.
[0019]The present invention in another embodiment provides a method of producing the detectably labeled porphyrin compound, comprising the steps of: preparing a stannous chloride solution; adding Tc-99m-sodium pertechnetate solution into the stannous chloride solution to create a mixture; stirring the mixture at room temperature; adding purified phthalocyanine tetrasulfonate (PCTS) to the mixture; stirring the mixture again at room temperature; incubating the mixture at room temperature; and passing the mixture through a filter.
[0020]The present invention in another embodiment provides a method of identifying a sentinel lymph node, comprising the steps of: injecting the detectably labeled porphyrin compound into a tumor, wherein the porphyrin compound is detectably labeled with a radiolabel which is a γ-emitting radioactive metal; and detecting radioactive emission or a color so as to detect and thereby identify the sentinel lymph node.
[0021]The present invention in another embodiment provides a method of identifying a sentinel lymph node, comprising the steps of: injecting the detectably labeled porphyrin compound into tissue surrounding a tumor, wherein the porphyrin compound is detectably labeled with a non-radioactive metal; and taking a series of magnetic resonance images to identify the sentinel lymph node by viewing the images showing the injected detectably labeled porphyrin compound.

Problems solved by technology

This causes problems relating to the accuracy of the identification.
There is a disadvantage to lymphazurin blue in that it cannot be radio-labeled.
Another problem is that not only does the procedure require two substances, it also requires two separate injections.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Tc99m-PCTS

[0035]Into a freshly prepared stannous chloride solution (0.38 mg, 0.02 mmole / 100 ul of 0.1 N Hcl) was added Tc-99m-sodium pertechnetate solution (30.0 mCi / 200 ul of normal saline). The mixture was stirred magnetically at room temperature for two minutes. Into this mixture was added 2 ml of phthalocyanine tetrasulfonate (PCTS), pH 8.2, solution (100.2 mg, 0.12 mmole / 2 ml) (TSW-V-135). The mixture was stirred at room temperature for five minutes, and then incubated at room temperature for thirty minutes. The product was passed through a 0.22 um filter. The radioactivity yield of the product prepared was 29.22 mCi (97.4%). The pH of the product was 7.8.

[0036]This next section describes the quality control of the above prepared solution. The radioactivity purity of the product was assayed by radiochromatography with whatman #1 paper and Instant Thin Layer Chromatography-silica gel (ITLC-SG) system and developed in acetone. One drop of sample was spotted on the ...

example 2

Palpable Tumor Injection

[0040]The first scenario is a palpable tumor. In this scenario, the radio-labeled blue porphyrin acting as a dye is injected into the breast tissue surrounding the lesion. The injection sites are delineated by palpating the tumor and are usually located at 3, 6, 9 and 12 o'clock of the lesion. A 25-gauge needle may be used for the injection. The breast is massaged for at least five minutes to facilitate the flow of the dye into the lymphatics.

[0041]A pre-operative lympho-scintigram may be performed as early as five minutes following the injection to delineate the lymph node basin draining the radio-labeled substance. Alternatively, a gamma probe detector is used to locate and identify the sentinel lymph node. The gamma probe detector is attached to a signal processor that allows the surgeon to detect the amount of radioactivity by way of a digital readout or an audio sound. The highest point of radioactivity detected at a site different from the tumor is iden...

example 3

Malignant Lesion Injection

[0042]The second scenario is a malignant lesion detected by a mammogram or any other diagnostic modality such as ultra sound, magnetic resonance imaging, or others.

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PUM

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Abstract

A detectably labeled porphyrin compound for identifying a sentinel lymph node in patients, particularly those with cancer. A method of identifying a sentinel lymph node, comprising the steps of: injecting the detectably labeled porphyrin compound into a tumor, wherein the porphyrin compound is detectably labeled with a radiolabel which is a γ-emitting radioactive metal; and detecting radioactive emission or a color so as to detect and thereby identify the sentinel lymph node. A method of identifying a sentinel lymph node, comprising the steps of injecting the detectably labeled porphyrin compound into tissue surrounding a tumor, wherein the porphyrin compound is detectably labeled with a non-radioactive metal; and taking a series of magnetic resonance images to identify the sentinel lymph node by viewing the images showing the injected detectably labeled porphyrin compound.

Description

[0001]This application is a divisional of U.S. Ser. No. 09 / 583,359, filed May 30, 2000 now U.S. Pat. No. 6,458,336.[0002]Throughout this application, various publications are referenced to by arabic numerals within parentheses. Disclosures of these publications in their entireties are hereby incorporated by reference into this application to more fully describe the state of the art to which this invention pertains. Full bibliographic citations for these references may be found at the end of this application, preceding the claims.FIELD OF THE INVENTION[0003]The present invention relates to a substance and method for identifying the sentinel lymph node in patients, particularly those with cancer. More particularly, the present invention relates to a detectably labeled porphyrin compound for identifying the sentinel lymph node.BACKGROUND OF THE INVENTION[0004]One of the major techniques for determining the prognosis of cancer, particularly breast cancer, involves examining the lymph no...

Claims

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Application Information

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IPC IPC(8): A61B5/055A61K51/04
CPCA61K51/0485
Inventor EL-TAMER, MAHMOUDFAWAZ, RASHIDWANG, THEODORECHAGLASSIAN, TEDSAOUAF, ROLA
Owner THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK
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