Calcium containing chitosan stent material

Abstract

The invention discloses a scaffold material of chitosan calcic liquid colloid. The scaffold material of the invention is prepared by the steps that chitosan particles precisely weighed and sub-packaged are radiated for 1000min by 10K Gy Co<60> for sterilization; 0.1 percent of acetic acid is served as a solvent to be made into 1.2 percent of water solution; 0.108g of tricalcium phosphate powder and 5 percent of gelatin granules are added and evenly blended under the constant stirring by a magnetic rotor in the water bath of 60 DEG C; 1M NaOH solution titration is added until the pH is 7 after the still standing and cross-linking for 1.5h. The scaffold material of chitosan calcic liquid colloid of the invention is used for being injected into a bone bioreactor at the subperiosteum cavity in a human body to induce osteogenesis and has good biocompatibility, strong flexibility, good quality of osteogenesis, low cost and great potential in bone transplant with simple operation without being cultured by external cells and adding cell factors or growth factors, is absorbable in the human body, and also ensures no risks of infection, immune rejection and the like. In addition, the end product of the metabolism of glucose can also provide energy for series osteoblastic responses. Therefore, the invention provides the good scaffold material for the bone bioreactor.

Description

technical field

[0001] The invention relates to an in vivo bone bioreactor induced osteogenesis material for bone regeneration and bone defect repair, in particular to an absorbable and injectable chitosan calcium-containing colloid scaffold material. Background technique

[0002] The repair of long bone and flat bone defects caused by congenital, trauma, inflammation and tumors has long been a difficult problem for clinicians. How to obtain a sufficient amount of donor bone for transplantation to fill bone defects, while meeting the long-term requirements for shape, strength, and / or load-bearing of the bone in the defect recipient area, without causing immune rejection has been a research hotspot for a long time.

[0003] Autologous bone grafting is a traditional bone defect repair method commonly used in clinic. The bone donor sites include ilium, fibula, ribs, skull, etc., and can provide cancellous bone, cortical bone and whole bone. Because of autologous transplantatio...

Examples

Embodiment Construction

[0018] In order to further illustrate the chitosan calcium-containing liquid colloid scaffolding material of the present invention, the following examples will be described in more detail.

[0019] Chitosan (Chitosan), the chemical name is β(1,4)2-amino 2-deoxy-D protoglycan, which is the deacetylation product of methyl ketone, and its content in natural polymers is second only to cellulose. It is also the only natural alkaline polysaccharide with unique molecular structure, chemical properties and biological activity.

[0020] Previous literature shows that chitosan has good biocompatibility, that is, it will not produce systemic or local toxic reactions, will not cause hemolysis, will not cause inflammation and rejection, will not cause mutations, and is conducive to the rapid growth of bone tissue into the pores of the material. And tightly combined with the material. The porous chitosan scaffolds were implanted into the mice, and the mice were killed at 1, 2, 4, 8 and 12 ...

technical field

[0001] The invention relates to an in vivo bone bioreactor induced osteogenesis material for bone regeneration and bone defect repair, in particular to an absorbable and injectable chitosan calcium-containing colloid scaffold material. Background technique

[0002] The repair of long bone and flat bone defects caused by congenital, trauma, inflammation and tumors has long been a difficult problem for clinicians. How to obtain a sufficient amount of donor bone for transplantation to fill bone defects, while meeting the long-term requirements for shape, strength, and / or load-bearing of the bone in the defect recipient area, without causing immune rejection has been a research hotspot for a long time.

[0003] Autologous bone grafting is a traditional bone defect repair method commonly used in clinic. The bone donor sites include ilium, fibula, ribs, skull, etc., and can provide cancellous bone, cortical bone and whole bone. Because of autologous transplantatio...

Examples

Embodiment Construction

[0018] In order to further illustrate the chitosan calcium-containing liquid colloid scaffolding material of the present invention, the following examples will be described in more detail.

[0019] Chitosan (Chitosan), the chemical name is β(1,4)2-amino 2-deoxy-D protoglycan, which is the deacetylation product of methyl ketone, and its content in natural polymers is second only to cellulose. It is also the only natural alkaline polysaccharide with unique molecular structure, chemical properties and biological activity.

[0020] Previous literature shows that chitosan has good biocompatibility, that is, it will not produce systemic or local toxic reactions, will not cause hemolysis, will not cause inflammation and rejection, will not cause mutations, and is conducive to the rapid growth of bone tissue into the pores of the material. And tightly combined with the material. The porous chitosan scaffolds were implanted into the mice, and the mice were killed at 1, 2, 4, 8 and 12 ...