Described herein are
proteases of any of SEQ ID NOs: 6-14 or a proteolytically active variant thereof with greater than 85%, 90%, 95% or 98%
sequence identity thereto, wherein the proteolytically active variant cleaves
a peptide of SEQ ID NO: 17 at QP|QL to provide peptides of any of SEQ ID NOs. 18-24. Also described are compositions such as pharmaceutical compositions, food supplements, and
food products including the
proteases. Further described are methods of treating a disorder associated with
gluten intolerance by administering to a subject in need thereof an effective amount of the
proteases. Also included are methods of testing a subject suspected of having
gluten intolerance or
gluten sensitivity by administering a composition including a
protease for a period of time, then assessing
symptom improvement in the subject.