The invention belongs to the field of medicine and health care products, and particularly relates to a composition with speckle lightening and removing effects, a preparation method and application thereof. The invention aims to solve the technical problem of providing the brand new composition with speckle lightening and removing effects, in the basic prescription, the active ingredients of the composition are composed by the following raw medicines in parts by weight: 600-1000 parts of astragalus mongholicus, 300-700 parts of angelica sinensis and 200-600 parts of safflower carthamus; and in the optimized prescription, the active ingredients of the composition are composed by the following raw medicines in parts by weight: 600-1000 parts of astragalus mongholicus, 300-700 parts of angelica sinensis, 200-600 parts of safflower carthamus, 60-100 parts of aloe and 40-100 parts of grape seed extract. The application of the composition can realize the speckle lightening and removing and whitening facial effects. Human experiments show that: after the composition is taken for 1-3months, speckles are lightened, the removing effect is obvious, and the composition can be used for chloasma, freckles, age pigment, sunburn and the like, and has particularly obvious effect on curing the chloasma.