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The application relates to the field of biotechnology, and particularly discloses a mutant strain Cbg61-T37, a co-expression strain Cbg61-T37-MPR1 and a construction method and application thereof. The mutant strain Cbg61-T37 is preserved in the China General Microbiological Culture Collection Center on August 18, 2025, has a preservation number of CGMCC No. 35649, and is classified and named as Pichia pastoris. Pichia pastoris The mutant strain Cbg61-T37 provided by the application has an enzyme hydrolysis activity of 62.9 U / mg, which is increased by 43.8% compared to the original strain. Therefore, the mutant strain Cbg61-T37 is more conducive to catalysis and synthesis of the rare ginsenoside CK.

The invention relates to agriculture. The liquid complex fertilizer containing colloidal silver and a complex of macro- and microelements in the form of salt, as well as in the form of chelates - metal-ethylenediaminetetraacetic acid, while it additionally contains sodium tallow amphopolycarboxyglycinate, polyvinylpyrrolidone, water, pre-structured with shungite. All components are taken at a certain ratio. The invention makes it possible to increase the following: acaricidal activity against the common spider mite, the insecticidal activity against the common potato aphid, the antiviral activity against the tomato yellow leaf curl virus, and also the stability of the fertilizer as a colloidal system.