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The application relates to the field of biotechnology, and particularly discloses a mutant strain Cbg61-T37, a co-expression strain Cbg61-T37-MPR1 and a construction method and application thereof. The mutant strain Cbg61-T37 is preserved in the China General Microbiological Culture Collection Center on August 18, 2025, has a preservation number of CGMCC No. 35649, and is classified and named as Pichia pastoris. Pichia pastoris The mutant strain Cbg61-T37 provided by the application has an enzyme hydrolysis activity of 62.9 U / mg, which is increased by 43.8% compared to the original strain. Therefore, the mutant strain Cbg61-T37 is more conducive to catalysis and synthesis of the rare ginsenoside CK.

The present invention belongs to the field of microbial technology, and specifically relates to a strain of Lactobacillus crispatus NCU‑13 and its application. The name of the Lactobacillus crispatus NCU‑13 is Lactobacillus crispatus NCU‑13, which has been deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee on July 17, 2023, with a deposit number of CGMCC No.27918. The present invention evenly places the freeze-dried Lactobacillus crispatus powder in the absorption layer of the sanitary napkin, and when in use, utilizes the suitable environment of the human body and the moist environment formed during menstruation to activate and promote the activation and proliferation of probiotics attached to the sanitary napkin, so that it enters the vagina. The Lactobacillus crispatus and its beneficial metabolites can not only promote the health of the vaginal environment, but also improve the barrier function of the vaginal mucosa, and may help restore the balance of vaginal microecology through its anti-inflammatory and immunomodulatory effects, while optimizing female reproductive health.

The utility model discloses a bolt and nut assembling device which comprises an operating platform, a bolt body, a nut body, a transverse moving mechanism, a height adjusting mechanism and a lubricating spraying mechanism. The transverse moving mechanism comprises a first sliding plate, a first sliding rail, a second sliding rail, a second sliding plate and an operating handle; the height adjusting mechanism comprises a supporting rod, a first adjusting frame, a second adjusting frame and an adjusting rod, the lubricating spraying mechanism comprises a transverse plate, a lubricating oil tank, a connecting pipe, a sprayer and a spraying pipe, and due to the fact that a first sliding plate and a second sliding plate are installed on a first sliding rail and a second sliding rail, the first sliding plate and the second sliding plate are allowed to smoothly move along the rails; the flexibility and operability of the manipulator are improved, the height of a bolt body and the height of a nut body can be adjusted at the same time through the two height adjusting mechanisms, it means that the device can meet the working requirements of different heights, the bolt body is lubricated through the spraying mechanism, and friction in the assembling process can be remarkably reduced.

The invention relates to agriculture. The liquid complex fertilizer containing colloidal silver and a complex of macro- and microelements in the form of salt, as well as in the form of chelates - metal-ethylenediaminetetraacetic acid, while it additionally contains sodium tallow amphopolycarboxyglycinate, polyvinylpyrrolidone, water, pre-structured with shungite. All components are taken at a certain ratio. The invention makes it possible to increase the following: acaricidal activity against the common spider mite, the insecticidal activity against the common potato aphid, the antiviral activity against the tomato yellow leaf curl virus, and also the stability of the fertilizer as a colloidal system.