Process for industrialized production of microsporidian using silkworm as host

A technology of microsporidia and industrialization, which is applied in the direction of protozoa, etc., to achieve the effect of large output, large individual and difficult source

Inactive Publication Date: 2008-05-21
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no research reports on the use of silkworms to reproduce microsporidia in large numbers for biocontrol applications in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Propagation of Microsporidia M-Px by Bombyx mori

[0017] Selection of silkworm eggs: Select healthy silkworm eggs without microsporidia germ infection as the breeding host.

[0018] Raising conditions: the raising temperature of silkworm larvae is 25-30° C., the humidity is 70-85%, natural light, and fresh normal mulberry leaves are raised.

[0019] Inoculation dose and period: The purified M-Px spore liquid was prepared to a concentration of 2×10 6 each / mL, applied on the surface of mulberry leaves, fed silkworms continuously for 8 hours from the 3rd instar, and then replaced with fresh and normal mulberry leaves for normal feeding, and reared until pupation and moths, collected dead insects and moths, and kept in the refrigerator for 5 Store at ℃.

[0020] Collection and purification of microsporidia: Take out the dead insects and moths stored in the refrigerator, grind them with a grinder, filter them with nylon gauze, and centrifuge them at differential...

Embodiment 2

[0021] Example 2 Propagation of Microsporidia M-Le by Bombyx mori

[0022] Selection of silkworm eggs: Select healthy silkworm eggs without microsporidia germ infection as the breeding host.

[0023] Breeding conditions: Bombyx mori larvae are raised at a temperature of 25-30° C., humidity of 70-85%, natural light, and fresh normal mulberry leaves.

[0024] Inoculation dose and period: The purified M-Le spore liquid was prepared to a concentration of 1×10 6 each / mL, applied on the surface of mulberry leaves, fed silkworms continuously for 8 hours from the 3rd instar, and then replaced with fresh and normal mulberry leaves for normal feeding, and reared until pupation and moths, collected dead insects and moths, and kept in the refrigerator for 5 Store at ℃.

[0025] Collection and purification of microsporidia: Take out the dead insects and moths stored in the refrigerator, grind them with a grinder, filter them with nylon gauze, and centrifuge them at differential speed for...

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PUM

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Abstract

This invention disclses a production technique of factory-producting microsporozoon, which uses cultivated silkworm as the host. The microsporozoon has the specificity of being very wide to parasitic insects. In this technique we choose cultivated silkworm as substitute because its feeding technique is maturate, and it is easy to feed and propagate, and it can be feed by many times all through a year. Artificially creat infected sickness condition to achieve mass reproduction of the aimed microsporozoon. The hos cultivated silkworm has such advantages as big body, mass yield, low feeding cost, scalizable feeding, industrializable producting, consequen- tly the resource difficulty of microsporozoon is resolved.

Description

technical field [0001] The invention relates to the research on biological control of pests, in particular to a process for industrially producing microsporidia M-Px or M-Le by using silkworm as a host. Background technique [0002] Microsporidia are obligate intracellular parasites, which can not only cause disease and death of host insects, but also most microsporidia can infect offspring through female embryos, resulting in the death of offspring. This vertical transmission can save Pathogenic species can continue to expand and spread to form epidemics. This is a remarkable feature that other entomopathogenic microorganisms such as bacteria and fungi do not have. From the perspective of controlling the number of pest populations, it has special significance. Therefore, microsporidia are used as insecticidal microbial pesticides, with high insecticidal efficacy and long control time. They are a type of microbial pesticide resources with great potential and application pros...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/10
Inventor 杨琼郑祥明方定坚廖森泰黄炳辉邹宇晓蔡月仙罗国庆吴福泉唐翠明李夫涛刘新涛
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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