Sheep pox inactivated vaccine and preparation method thereof

An inactivated vaccine and sheeppox technology, applied in the field of inactivated sheeppox vaccine and its production, can solve the problems of needing intradermal immunization and difficult operation of live sheeppox vaccine, and achieve the effect of avoiding biosafety risks.

Inactive Publication Date: 2019-01-15
CHINA INST OF VETERINARY DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, this vaccine can be immunized by subcutaneous or intramuscular injection in the neck, which solves the difficult problem that live sheeppox vaccine needs intradermal immunization and is difficult to operate in the process of clinical use

Method used

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  • Sheep pox inactivated vaccine and preparation method thereof
  • Sheep pox inactivated vaccine and preparation method thereof
  • Sheep pox inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] —— Vaccine preparation and testing

[0038] According to the established production process, three batches of inactivated sheeppox vaccine were prepared, and the results are reported as follows.

[0039] 1. Materials

[0040] (1) The cells used for cell production are BHK-21 suspension cell strains, which are preserved by the Institute (see China Veterinary Drug Administration, China Veterinary Microbial Cultures Preservation Management Center, Chinese Veterinary Strain Catalog, Chinese Agricultural Science and Technology Press, 2008, p147).

[0041] (2) Virus sheep pox virus AV41 strain and AV40 strain.

[0042] (3) Oil adjuvant Montanide ISA 201 adjuvant, produced by SEPPIC, France.

[0043] (4) Experimental animals: 3-month-old healthy and susceptible Inner Mongolia cashmere goats, free from infectious diseases, skin diseases and parasitic infections. Blood was collected before immunization to determine the neutralizing antibody titer of goat pox virus, and sheep...

Embodiment 2

[0073] ——Comparative research report with similar products

[0074] In order to compare the differences in safety, efficacy and immune production period between the inactivated sheeppox vaccine (AV41 strain, suspension culture) and similar products, three batches of trial-produced sheeppox inactivated vaccine (AV41 strain, suspension culture) products and domestic Similar products, compare the safety and efficacy of two vaccinations (21-day challenge protection rate after immunization).

[0075] 1 Materials and methods

[0076] 1.1 Materials

[0077] 1.1.1 Experimental vaccines Three batches of goat pox inactivated vaccines: batch numbers 201601, 201602 and 201603 (30 bottles each), prepared by our laboratory.

[0078] 1.1.2 Control vaccine Goat pox live vaccine, batch number 1605001, produced by China Animal Husbandry Co., Ltd. Lanzhou Biopharmaceutical Factory.

[0079] 1.1.3 AV40 strain, F3 generation, 1.0g / bottle, is identified, kept and supplied by China Veterinary Dru...

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Abstract

The invention relates to a sheep pox inactivated vaccine and preparation method thereof. The invention adopts AV41 strain of goat pox virus to inoculate BHK-21 cell, harvesting culture, concentrate, purifying, inactivating BEI, adding suitable adjuvant, mixing and emulsifying. Used to prevent goat pox and sheep pox. The suspension culture BHK-21 cell produce an inactivated sheep pox vaccine, compared with the live vaccine prepared from sheep testicular cells, The present invention not only solves the problem that sheep testicles are difficult to obtain in the production process of live sheep pox vaccine, but also avoids the biological safety risk of primary generation cells carrying exogenous virus, and the prepared live sheep pox vaccine has no potential safety hazards such as lamb death,ewe abortion and the like. In addition, the vaccine can be injected subcutaneously or intramuscularly through the neck to solve the problem of intradermal immunization of live sheep pox vaccine, which is difficult to operate in clinical use.

Description

technical field [0001] The invention relates to an inactivated sheeppox vaccine and a production method thereof. It belongs to the field of veterinary biological products. Background technique [0002] The sheep raising industry is closely related to the development of the national economy and the improvement of people's living standards. Especially after entering the 21st century, my country's sheep raising industry has developed rapidly with remarkable results, and the proportion of the sheep raising industry in the national economy has also increased year by year. According to the data of the Food and Agriculture Organization of the United Nations (FAO), in 2013, the number of sheep breeding in mainland China reached 191 million heads and 183 million goats, totaling 374 million heads. In 2014, there were 202 million sheep and 188 million goats in mainland China, totaling 390 million. Although the number of sheep raised in my country ranks first in the world, compared wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/275A61K39/39A61P31/20
CPCA61K9/0019A61K39/12A61K39/39A61K2039/5252A61K2039/552A61K2039/55566A61P31/20C12N2710/24234
Inventor 陈小云杜吉革王磊王静文张敏薛麒朱真
Owner CHINA INST OF VETERINARY DRUG CONTROL
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