Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Full cell catalyst of intracellular secretory lipase type and its preparing method

A whole-cell catalyst and endocrine technology, applied in the fields of biochemical equipment and methods, enzymes, hydrolase, etc., can solve the problems of repeated use and other problems, and achieve the effect of saving equipment investment, simple production, and easy to use multiple times.

Inactive Publication Date: 2009-01-28
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, Zeng Jing et al. from Tsinghua University used molds to dry and freeze directly to participate in the reaction practice, but there were no reports of repeated use.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method for intracellular secreted lipase-type whole-cell catalyst, which comprises the steps of:

[0023] 1). Selection of strains and culture medium: (1) Strains: the waste oil from the oil factory was placed in the medium for separation by the dilution plate method for separation and culture. The medium for separation was potato sucrose medium, and the cells produced Yeast (Candidalipolytica lipolytica) of endocrine lipase (see "Agricultural Microbiology Experimental Technology" page 69-72 for the isolation method, China Agricultural Press, May 1996, first edition); wherein potato sucrose medium Method: cut 200g potatoes into small pieces and boil them into juice, dissolve potato juice and 20g sucrose in 1L water, add 20g agar, sterilize at 121°C for 15min, and obtain potato sucrose medium; (2) Fermentation medium : Soybean oil 29.0g / L, sucrose 1.0g / L, peptone 70.0g / L, NaNO 3 1.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, sterilized at 121°C for ...

Embodiment 2

[0031] A preparation method for intracellular lipase-type whole-cell catalyst, which comprises the steps of:

[0032] 1). Selection of strains and culture medium: (1) Strains: the waste oil from the oil factory was placed in the medium for separation by the dilution plate method for separation and culture. The medium for separation was potato sucrose medium, and the cells produced Yeast (Candidalipolytica lipolytica) of endocrine lipase (see "Agricultural Microbiology Experimental Technology" page 69-72 for the isolation method, China Agricultural Press, May 1996, first edition); wherein potato sucrose medium Method: cut 200g potatoes into small pieces and boil them into juice, dissolve potato juice and 20g sucrose in 1L water, add 20g agar, sterilize at 121°C for 15min, and obtain potato sucrose medium; (2) Fermentation medium : Soybean oil 29.0g / L, sucrose 1.0g / L, peptone 70.0g / L, NaNO 3 1.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, sterilized at 121°C for 20 minute...

Embodiment 3

[0040] A preparation method for intracellular lipase-type whole-cell catalyst, which comprises the steps of:

[0041] 1). Selection of strains and culture medium: (1) Strains: the waste oil from the oil factory was placed in the medium for separation by the dilution plate method for separation and culture. The medium for separation was potato sucrose medium, and the cells produced Yeast (Candidalipolytica lipolytica) of endocrine lipase (see "Agricultural Microbiology Experimental Technology" page 69-72 for the isolation method, China Agricultural Press, May 1996, first edition); wherein potato sucrose medium Method: cut 200g potatoes into small pieces and boil them into juice, dissolve potato juice and 20g sucrose in 1L water, add 20g agar, sterilize at 121°C for 15min, and obtain potato sucrose medium; (2) Fermentation medium : Soybean oil 29.0g / L, sucrose 1.0g / L, peptone 70.0g / L, NaNO 3 1.0g / L, KH 2 PO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, sterilized at 121°C for 20 minute...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The present invention relates to one kind of full cell catalyst with lipase activity and its preparation process. The intracellular lipase secreting full cell catalyst is prepared with the materials including saccharomycetes 0.7-1.2 wt%, diatomite 2.1-3.6 wt%, sodium alginate 2.0-2.3 wt%, calcium dichloride 0.5-0.8 wt%, and pH 7.7 Na2HPO4-NaH2PO4 buffer solution of 92.1-94.7 wt%, with the saccharomycetes to diatomite weight ratio being 1 to 3. The intracellular lipase secreting full cell catalyst is used to replace lipase in producing biological diesel oil, and has simple preparation process, easy product recovery, reusability and low production cost.

Description

technical field [0001] The invention relates to a preparation method of a whole cell catalyst with lipase activity. Background technique [0002] In the production of biodiesel, the biggest obstacle to the large-scale production of lipase as a catalyst is the high production cost of lipase, which makes it difficult to realize large-scale industrial production and application. Biological whole cells producing lipase are used as catalysts (referred to as whole cell catalysts). After the lipase-producing microbial strains are fermented and cultured, the bacteria are collected and replaced by lipase after simple treatment to participate in the reaction of synthesizing mono-fatty acid methyl esters. The whole-cell catalyst eliminates the complicated processes of separation, extraction, purification and immobilization of lipase, and is one of the effective ways to overcome the current high cost of lipase production and application. [0003] Biological whole-cell catalysts for lip...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N11/00C12N9/20
Inventor 江木兰胡小加晏立英张银波
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com