Green trichodermin and its use in production of Pu'er tea
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A technology of Trichoderma viride and Pu-erh tea is applied in the biological field to achieve the effects of stabilizing and improving quality, shortening processing time and improving quality
Inactive Publication Date: 2009-07-15
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology
However, how to improve the quality of Pu’er tea through the research of microorganisms in Yunnan Pu’er tea processing, and screening beneficial and dominant microbial strains from Pu’er tea processing for production has not yet been published in the literature.
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Embodiment 1
[0012] Embodiment 1: Purification of PTri050102 bacterial classification
[0013] From the collected Pu'er tea samples, Trichoderma viride strains were obtained by separation on a known plate. Trichoderma viride purification medium is: 200g potato, 20g glucose, 20g agar, 1000ml distilled water, natural pH. Peel the potatoes, cut them into pieces, cook them for half an hour, then filter them with gauze, add agar and sugar, melt them and add water to 1000ml. Sterilize at 121°C for 20 minutes.
[0014] Purification method:
[0015] (1) Melt the known potato dextrose agar medium, cool it to 45°C, pour it into a sterile petri dish, about 15-20 milliliters per dish, shake slightly, let it stand, and make it into a flat plate for later use.
[0016] (2) Take 2g of the tea sample to be separated, add it to a mortar with 98ml of sterile water beside the flame and grind it, and what is obtained at this time is 10 -2 bacterial suspension.
[0017] (3) Dilution: Dilute the bacterial ...
Embodiment 2
[0021] Embodiment 2: the production application of Trichoderma viride
[0022] The maocha is moist-heated according to the traditional method, and then 0.1-0.8% by weight of the PTri050102 strain is added to the maocha to ferment it. When the pile temperature rises to 30°C, the PTri050102 strain begins to metabolize, and with As the pile temperature rises further, a large amount of enzymes are secreted. These enzymes oxidize and hydrolyze the ingredients contained in tea leaves. Under the action of protease, the non-digestible macromolecular protein is degraded into peptone, polypeptide and various amino acids. Under the action of glucoamylase and cellulase, the macromolecular insoluble substances of the fermentation substrate are converted into small molecular substances soluble in water. The Pu-erh tea produced after inoculation has a unique aroma, mellow taste and sweet aftertaste. Tan oily quality characteristics.
[0023] The quality of Pu'er tea produced after being i...
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Abstract
The invention relates to a viride trichoderma fungus and its application in the production of Pu'er tea, which belongs to the field of biotechnology. The Trichoderma viride fungus of the present invention is obtained after the traditional primary screening, re-screening, natural medium culture, and Trichoderma purification and cultivation process by the production strain and auxiliary materials. The production strain is Trichoderma viride (Trichoderma viride) PTri050102, which is preserved in General Microbiology Center of China Microbiological Culture Collection Management Committee, the address of the preservation unit: No. 13, North Yiyi, Zhongguancun, Haidian District, Beijing, the preservation date: March 1, 2007, and the preservation number: CGMCC No.1962. The viride trichoderma fungus has the function of improving the quality of Pu'er tea, and can be applied in the production process of Pu'er tea. The present invention has the advantages that: when Trichoderma viride is used in the large-scale production process of Pu'er tea in a proportion of 0.1-0.8% by weight, the tea polyphenols and theabrownin in the Pu'er tea can be controlled at an appropriate ratio to reach The effect of improving quality and shortening the processing time plays an important role in stabilizing and improving the quality of Pu-erh tea.
Description
Technical field: [0001] The invention relates to a viride trichoderma fungus and its application in the production of Pu'er tea, which belongs to the field of biotechnology. Background technique: [0002] Trichoderma viride can produce cellulase, cellobiase, amylase, lactase and so on. It can be used to produce citric acid, can synthesize riboflavin and can be used for steroid conversion, and is also a type of fungus that produces antibiotics. The bacteria can be used in a wide range. [0003] During the large-scale production of Pu'er tea, the strain can secrete enzymes to act on the substrate. It can inhibit the formation of other miscellaneous bacteria, and can cooperate with other bacteria to play a good role in promoting the formation of "mellow and harmonious" quality of Pu'er tea. [0004] In the field of Pu-erh tea research, due to the long-term lack of subject research, especially from the perspective of microorganisms to study Pu-erh tea. However, how to improve...
Claims
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