Liver cancer cell specificity internalization short peptide and its in vitro screening and identification

Abstract

This invention relates to the special short peptide of hepatoma cell internalization and its screen and identification in vitro, belonging to the technology of tumor cell peptides screening and identification. It takes hepatoma cell BEL-7404 as the screening target cell, displays the random 12-peptide library for bacteria invader to conduct affinity panning. Through three times of screen, it randomly selects twenty negative colonies for amplification and sequence, to deduct the amino acid sequence of random peptide. Through cell ELISA, immunofluorescence, flow cytometry and other method, it further identifies the combination and internalization of bacteria invader clones and hepatoma cells. From sequencing and sequence analysis of the positive clones, four obtained different sequences have good combination with hepatoma cells, and the screened monoclone can internalize cells.

Description

technical field [0001] The invention relates to specific internalized short peptides of liver cancer cells and their in vitro screening and identification, belonging to the technical field of screening and identification of tumor cell specific internalized short peptides. Background technique [0002] Liver cancer is a serious threat to human health due to its extremely high mortality rate. In recent years, with the in-depth study of tumor biology and cell surface receptors, guided therapy has shown good therapeutic effects, and has gradually developed into an important field of tumor treatment. Targeted therapy refers to the use of targeting molecules that can specifically act on tumor tissues or organs as drug carriers to direct drugs to tumors, effectively improving the specificity of drug targeting, and achieving targeted and safe treatment. The currently widely used targeting molecules are mainly monoclonal antibodies and their fragments. In recent ...

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Problems solved by technology

[0004] Traditional screening techniques usually screen directly against tumor cell-specific proteins or the surface of tumor cells, and the obtained targeting molecules also bind to the surface of tumor cells. Although it solves the effect of locally increasing the concentration of therapeutic drugs, it cannot effectively improve the therapeutic effect. The entry of drugs into tumor cells, and most drugs need to enter the interior of the cells to work best

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