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Transplanting method for breeding grouped seedling of fleshiness plant

A technology for succulent plants and tissue culture seedlings, which is applied in the field of transplanting succulent plant tissue culture seedlings, can solve the problems of perishability and low transplant survival rate, and achieve the effects of well-developed root system, improved transplant survival rate, and simple method

Inactive Publication Date: 2007-10-10
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

[0003] Succulents are a very popular ornamental plant in recent years, involving tens of thousands of plants such as Cactaceae, Liliaceae, Apricotaceae, Crassulaceae, Euphorbiaceae, Compositae, etc. Their propagation mainly depends on sowing and cuttings, but This can no longer meet the needs of the market, so it has been propagated through plant tissue culture technology now, but the succulent plant tissue culture seedlings grow in a relatively constant temperature, high humidity, closed and sterile environment, and there is basically no epidermal protective tissue. Layer cells contain more than 90% water, so when transplanting the tissue culture seedlings into the cultivation medium according to the conventional transplanting method, the external temperature is changeable, the bacteria are mixed, and the high humidity environment is easy to rot. The survival rate is not high, only about 30%

Method used

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Effect test

Embodiment 1

[0015] Embodiment 1 The method for transplanting this succulent plant tissue culture seedling, the specific steps are as follows:

[0016] 1) Take out the tissue-cultured seedlings from the bottle: carefully take out the succulent plant tissue-cultured seedlings from the culture bottle, and wash the culture medium at the roots of the tissue-cultured seedlings with tap water;

[0017] 2), tissue culture seedling sterilization: dip the tissue culture seedling in 500mg / L 50% carbendazim wettable powder solution;

[0018] 3) Dry the tissue culture seedlings: neatly arrange the tissue culture seedlings in a clean shallow dish, place them in a cool and ventilated place for 2 to 3 days, and dry them until the surface of the tissue culture seedlings shrinks slightly;

[0019] 4) Transplanting of tissue culture seedlings: Plant the tissue culture seedlings into a moist cultivation medium, the cultivation medium is a mixed matrix of peat and perlite, the volume ratio of peat and perlite...

Embodiment 2

[0021] Embodiment 2 This succulent plant tissue culture seedling transplanting method, the specific steps are as follows:

[0022] 1) Take out the tissue-cultured seedlings from the bottle: Carefully take out the tissue-cultured seedlings of the succulents Twelve Juan and soft-leafed species Shou and Treasure Grass from the culture bottle, and wash the culture medium at the roots of the tissue-cultured seedlings with tap water;

[0023] 2), tissue culture seedling sterilization: dip the tissue culture seedling in 500mg / L 50% carbendazim wettable powder solution;

[0024] 3) Dry the tissue culture seedlings: neatly arrange the tissue culture seedlings in a clean shallow dish, put them in a ventilated and cool place for 3 days, and dry them until the surface of the tissue culture seedlings shrinks slightly;

[0025] 4) Transplanting of tissue culture seedlings: Plant the tissue culture seedlings into a moist cultivation medium, the cultivation medium is a mixed matrix of peat an...

Embodiment 3

[0027] Embodiment 3 The method for transplanting the tissue-cultured seedlings of succulent plants, the specific steps are as follows:

[0028] 1) Take out the tissue cultured seedlings from the bottle: Carefully take out the tissue cultured seedlings of the succulents Twelve Juan sclerites and the beautiful Twelve Juan tissue cultured plants from the culture bottle, and wash the culture medium at the root of the tissue cultured seedlings with tap water ;

[0029] 2), tissue culture seedling sterilization: dip the tissue culture seedling in 500mg / L 50% carbendazim wettable powder solution;

[0030] 3) Dry the tissue cultured seedlings: neatly arrange the tissue cultured seedlings in a clean shallow dish, put them in a ventilated and cool place for 2 days, and dry them until the surface of the tissue cultured seedlings shrinks slightly;

[0031] 4) Transplanting of tissue culture seedlings: Plant the tissue culture seedlings into a moist cultivation medium, the cultivation med...

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Abstract

A transplanting method for the tissue-cultured plantlet of pulpy plant includes such steps as taking the tissue-cultured plantlet out of the culture bottle, water washing for removing culture medium, dipping in the solution of wettable powder of carbendazim, drying in the air, transplanting it in the culture matrix, covering film for 7-10 days but opening the film for 1 hr in the morning and at night, and conventional transplanting and managing.

Description

technical field [0001] The invention relates to a technique for transplanting plant tissue culture seedlings, in particular to a transplanting method for succulent plant tissue culture seedlings. Background technique [0002] Utilizing plant tissue culture for rapid propagation technology is a commonly used plant seedling production method in flower gardening production. It is mainly divided into four production steps, namely the establishment of sterile seedlings, multiplication culture, rooting culture, and tissue culture seedling transplanting. Among them, the transplanting of tissue culture seedlings is a process from heterotrophic to autotrophic, from sterile to bacterial, from constant temperature, high humidity, and weak light to variable temperature, low humidity, and strong light. The technical requirements are very high. A little carelessness will reduce the survival rate of transplanting, thereby increasing the cost of seedling production. At present, the convent...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 牟豪杰徐刚汪一婷吕永平陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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