Extraction method for acute pancreatitis model animal pancreas tissue RNA
A technique for acute pancreatitis and model animals, which is applied in the field of molecular biology and can solve the problems of poor RNA extraction from pancreas tissue of animals with acute pancreatitis
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[0014] 1. Preparation of experimental drugs and disposal of experimental supplies
[0015] 1) Preparation of experimental drugs
[0016] Lavage fluid: HEPES 25mM, collagenase IV 40U / ml, soybean trypsin inhibitor 0.1mg / ml, NaCL 100mM, KCL 5mM, MgCL 2 1mM, CaCL 2 1mM, D-dextrose 14mM, glutamine 2mM, 2% (w / v) BSA, prepared with sterile saline, sterilized on the filter membrane in 95% O 2 , 5% CO 2 , Incubate at 37°C for 2 hours for later use.
[0017] Soybean trypsin inhibitor (0.2mg / ml): Prepared with sterile saline and stored at 4°C.
[0018] 75% ethanol: Prepared with RNase-free deionized water treated with DEPC and stored at 4°C.
[0019] TE solution: Use DEPC-treated deionized water without RNase to dilute the sterile packaged TE concentrate.
[0020] 2) Handling of experimental supplies
[0021] Deionized water was added to DEPC with a concentration of 0.1%, heated at 37°C for 12 hours, and subjected to high temperature treatment at 125°C for 2 times for 30 minutes each time to...
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