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Candida albicans infection mould and construction method thereof

A technology of Candida albicans, construction method, applied in the directions of botanical equipment and methods, biochemical equipment and methods, animal husbandry, etc., to achieve the effect of simple operation and easy observation

Inactive Publication Date: 2007-11-28
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of this paper is to provide a simple and easy-to-observe method for establishing an animal model of Candida albicans infection, so as to overcome the problems existing in the current animal model of Candida albicans infection

Method used

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  • Candida albicans infection mould and construction method thereof
  • Candida albicans infection mould and construction method thereof
  • Candida albicans infection mould and construction method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Candida albicans (Candida albicans) strains were inoculated on Sabouraud'sbroth (ingredients: glucose 40g, peptone 10g, agar 20g, dissolved in 1000ml distilled water), cultured at 37°C for 48 hours after inoculation, sterile Add sterile normal saline to the surface of the culture medium under certain conditions, pick colonies from sterile platinum ear, make it form a suspension with sterile normal saline, centrifuge, and use sterile normal saline to prepare 1×10 5 spores / ml bacterial solution, male rabbit weighing 3.75kg, skin on both sides of ear, left ear (avoiding ear arteries and veins) sterilized with 2% iodine and 75% alcohol, sterile injected 1.0ml, 24h later A local abscess infection of 2cm×3cm appeared.

Embodiment 2

[0025] With the method of Example 1, the Candida albicans strain was inoculated, cultivated, and formed a suspension with sterile saline, centrifuged, and prepared into 1 × 10 with sterile saline 5.5 1 spore / ml bacterial solution, inject 1.0ml in the left ear of a male rabbit weighing 4.05kg, and a local abscess infection of 2.5cm×3cm will appear after 24h.

Embodiment 3

[0027] With the method of Example 1, the Candida albicans strain was inoculated, cultivated, and formed a suspension with sterile saline, centrifuged, and prepared into 1 × 10 with sterile saline 6 1 spore / ml bacterial liquid, inject 1.0ml in the left ear of a male rabbit weighing 3.75kg, and local abscess infection of 3cm×4cm will appear after 24h.

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Abstract

An animal model infected by candida albicans where the primary infection is the local abscess in the ear and is accompanied by the infection of candida albicans in all organs. The method is to inject the candida albicans into the subcutaneous of the ear of a rabbit and the concentration of the candida albicans is 1X105 / ml-1X1010 / ml. By doing so, not only the local abscess in the ear is formed, but all organs are infected through blood circulation. In this invention, the model is easy to obtain; the local abscess in the ear can be observed conveniently; the two ears can be compared and the infection of all the organs can be detected at the same time. Thus, the present invention provides a simple and convenient animal model for developing new methods and medicines for the treatment of candida albicans.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an animal model of bacterial infection disease used for the pathogenic mechanism of bacteria, the screening of therapeutic drugs, the observation of the curative effect of drugs and the evaluation of the protective effect of vaccines. Background technique [0002] In recent years, with the general application of broad-spectrum antibiotics, hormones and immunosuppressants, as well as the radiotherapy and chemotherapy of tumor patients and the gradual increase of patients with other acquired immunocompromise (such as AIDS, etc.), the internal organs, skin and mucous membranes are infected. The number of fungal infections is increasing. Candida albicans is a common opportunistic pathogen. Its deep (systemic) infection is life-threatening, and the mortality rate remains high in the case of treatment with antifungal drugs. In recent decades, Candida albicans (Candida albicans) infection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/00A01K67/027
Inventor 刘为国
Owner DALIAN UNIV
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