Candida albicans infection mould and construction method thereof
A technology of Candida albicans, construction method, applied in the directions of botanical equipment and methods, biochemical equipment and methods, animal husbandry, etc., to achieve the effect of simple operation and easy observation
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Embodiment 1
[0023] Candida albicans (Candida albicans) strains were inoculated on Sabouraud'sbroth (ingredients: glucose 40g, peptone 10g, agar 20g, dissolved in 1000ml distilled water), cultured at 37°C for 48 hours after inoculation, sterile Add sterile normal saline to the surface of the culture medium under certain conditions, pick colonies from sterile platinum ear, make it form a suspension with sterile normal saline, centrifuge, and use sterile normal saline to prepare 1×10 5 spores / ml bacterial solution, male rabbit weighing 3.75kg, skin on both sides of ear, left ear (avoiding ear arteries and veins) sterilized with 2% iodine and 75% alcohol, sterile injected 1.0ml, 24h later A local abscess infection of 2cm×3cm appeared.
Embodiment 2
[0025] With the method of Example 1, the Candida albicans strain was inoculated, cultivated, and formed a suspension with sterile saline, centrifuged, and prepared into 1 × 10 with sterile saline 5.5 1 spore / ml bacterial solution, inject 1.0ml in the left ear of a male rabbit weighing 4.05kg, and a local abscess infection of 2.5cm×3cm will appear after 24h.
Embodiment 3
[0027] With the method of Example 1, the Candida albicans strain was inoculated, cultivated, and formed a suspension with sterile saline, centrifuged, and prepared into 1 × 10 with sterile saline 6 1 spore / ml bacterial liquid, inject 1.0ml in the left ear of a male rabbit weighing 3.75kg, and local abscess infection of 3cm×4cm will appear after 24h.
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