Reagent kit used for screening irregular antibody in blood serum and preparing method thereof

Abstract

The invention discloses an ELISA reagent box for checking irregular antibody in serum and the preparing way for the reagent. The reagent box comprises an enzyme link board, on which O mixed red cell membrane protein is wrapped, the membrane protein contains all red cell antigens; some negative serum, which is for negative comparison with human serum that has no agglutination reaction with the above red cells; and some positive serum, which is for positive comparison with human serum that has agglutination reaction with the above red cells. The principle of the invention is: no O spectrum red cell is used as detection reagent, in stead red cell membrane protein is extracted, wherein, the antigen containing red cell surface is wrapped on the enzyme link board; and enzyme linkage immunity analysis method (ELISA) is used to check whether a serum to be tested contains any irregular antibody. The advantages with the invention are: the long storage period, the high throughput, the easy to judge results and the simple detection method.

Description

technical field [0001] The present invention relates to an in vitro diagnostic reagent, more specifically, the present invention relates to an enzyme-linked immunosorbent assay (hereinafter abbreviated as ELISA) kit, which can simultaneously screen whether blood group irregular antibodies are contained in serum. Background technique [0002] Irregular antibodies in serum refer to blood group antibodies that do not conform to the Landsteiner rule of the ABO blood group system, that is, anti-A and anti-B blood group antibodies. The subtype, variant anti-A1 or certain anti-B antibodies in the ABO system are also called irregular antibodies, and most of them are IgG antibodies. These antibodies are mainly produced by immune stimulation such as blood transfusion or pregnancy. They cannot agglutinate in saline medium but can only sensitize red blood cells to the corresponding antigen. They must be detected by special methods (anti-human globulin method, polybrene method, etc.) Ag...

AI Technical Summary

Problems solved by technology

Due to the short shelf life of spectrum cells, if not applied in time, the product will expire and be wasted;

[0013] 2. The operation is complicated, especially for a large number of samples, the workload is too large, and the result interpretation is difficult;

[0014] 3. Due to the limited cell spectrum of spectrum cells, the current spectrum cells cannot completely distinguish antibodies with irregular blood group of red blood cells, so antibodies cannot be accurately detected;

[0015] 4. Unable to identify irregular antibody composition in certain diseases (such as autoimmune hemolytic anemia);

[0016] 5. The detection results cannot be saved, which is not conducive to the preservation of experimental data;

[0017] 6. The detection cost is too high

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