Method for manufacturing beverage containing antioxidant ingredients substance
An anti-oxidation and anti-oxidant technology, applied in food preparation, food science, application, etc., can solve problems such as inability to perform, enhance eyesight, etc., achieve the effects of reducing breakage, reducing DNA damage, and strengthening anti-oxidation effects
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Embodiment 1
[0060] The subjects were 23 healthy and non-smoking males aged 27-40. For two weeks, eat a normal diet but avoid vegetables high in carotenoids. For the third and fourth weeks, they drank 330cc of tomato juice containing 40mg of lycopene every day. In weeks five and six, they consumed 330ml of carrot juice containing 22.3mg of beta-carotene and 15.7mg of alpha-carotene. For the seventh and eighth weeks, consume 10g of dried spinach powder mixed with water or milk containing 11.3mg of Lutein.
[0061] During the study, blood was collected weekly and a 'Comet assay' was used to detect DNA damage in the peripheral blood glymphatic system. The results show that supplementation with tomato, carrot and spinach products can significantly reduce the breakage of internal bonds in the DNA of lymphocytes. (Pool-Zobel et al, Carcinogenesis 1997). See Figure 4 for its graph.
Embodiment 2
[0063] 37 healthy, non-smoking, postmenopausal women between the ages of 50 and 70 were randomly assigned to five groups. Each of the five groups was given a different mix of doses on each day. The first group: Beta-carotene, Lycopene, Lutein (each serving is 4mg), the other three groups are 12mg with only a single ingredient, the second group is Lutein 12mg, the third group is Beta-carotene 12mg, and the fourth group is Lycopene 12mg, the fifth group is the control group.
[0064] On the 57th day after taking carotene, each group had a considerable reduction in DNA damage rate compared with the first day. But on the fifteenth day, there was a significant difference in the first group that mixed three doses: Beta-carotene, Lycopene, and Lutein. (Xianfeng Zhao et al, Am J ClinNutr 2006)
Embodiment 3
[0066] Taking 20 healthy women with an average age of 25.2 years old, they were given a standard diet in the first week, and then an average of 8mg of Lycopene, 0.5mg of Beta-carotene and 11mg of Vitamin C were added every day for three weeks. Analyzes of carotenoids, vitamin C and vitamin E in the blood and lymph. "Comet assay" and HPLC analysis were used to detect the protection of lymphocyte DNA.
[0067] Results: Lycopene will increase the concentration of Lycopene in plasma and lymphocytes. In plasma, the concentration of vitamin C will increase by ~35% (P<0.05), and in lymphocytes by ~230% (P<0.005). Vitamin E was significantly reduced in plasma, but not in lymph glands. It is not obvious that vitamin E protects DNA from oxidative damage.
[0068] Conclusion: Beta-carotene and Lycopene can increase the biochemical availability of vitamin C. This effect is a result of the increase from different antioxidants. This is why vitamin C is chosen over vitamin E. (P Riso Eu...
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