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Reagent kit for detecting susceptibility of cancer of the lungs with SNPs of gene ERCC2 etc.

A kit and susceptibility technology, which is applied in the field of kits for detecting lung cancer susceptibility through SNPs of ERCC2 and other genes, can solve the problems of decreased repair ability, weakened performance of transcription factor complexes, and decreased transcription factors.

Inactive Publication Date: 2008-04-02
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A number of current studies have shown that the polymorphism of this site will weaken the performance of the transcription factor complex that ERCC2 participates in, thereby reducing the ability to repair DNA damage, and the ability of transcription factors will also decrease at the same time, resulting in a series of Occurrence of diseases such as lung cancer, prostate cancer, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Use of detection kits

[0030] Step 1: Extraction of DNA template

[0031] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0032] Step 2: Real-time quantitative PCR reaction

[0033] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0034] Sense primer 1: 5'-GTGTTAAGTGAGAAGGTGAT-3' (SEQ ID NO: 1) with a Tm value of 556°C

[0035] Antisense primer 1: 5'-AGGATAGCCAGGAAGAGAAA-3' (SEQ ID NO: 2) with a Tm value of 58°C

[0036] Sense primer 2: 5'-TTTGCCATTCACTGTGTTGG-3' (SEQ ID NO: 3) with a Tm value of 58°C

[0037] Antisense primer 2: 5'-ATCCTTGCTGCTATCATCA-3' (SEQ ID NO: 4) with a Tm value of 54°C

[0038] Sense primer 3: 5'-ACTCAGGAGTCACCAGGAA-3' (SEQ ID NO: 5) with a Tm value of 58°C

[0039] Antisense primer 3: 5'-TCTGTTCTTCTGCAGGAGGA-3' (SEQ ID NO: 6) with a Tm value of 58°C

[0040] Sense primer 4: 5'-ATCAA...

Embodiment 2

[0051] Example 2. Services that guide people to proactively prevent lung cancer

[0052] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0053] Step 1: DNA Extraction

[0054] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0055] Step 2: Genotyping Assays

[0056] Using the kit provided by the invention, the SNP site No. rs1048943 on the CYP1A1 gene, the SNP site No. rs1136410 on the PARP1 gene, the SNP site No. rs13181 on the ERCC2 gene and the SNP site No. rs1799793 on the ERCC2 gene Fluorescent quantitative PCR detection was performed at the same time to determine the genotypes of the four SNPs loci.

[0057] Step 3: Instruct people to be proa...

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Abstract

The invention discloses a regent box for detecting the susceptibility to lung cancer, comprising a specific primer and a specific fluorescent probe which are capable of synchronously detecting an rs1048943 SNP locus on a CYPIA1 gene, an rs1136410 SNP locus on an PARP1gene, an rs13181 SNP locus and an rs1799793 SNP locus on an ERCC2 gene, and common components for fluorescent quantitative detection of PCR. The reagent box of invention predicts the susceptibility to lung cancer of an individual by detecting the genotype of genes on the SNP loci of the CYPIA1 gene, the PARP1 gene and the ERCC2 gene of the individual.

Description

technical field [0001] The present invention relates to a kit for detecting susceptibility to disease, especially a kit for detecting susceptibility to lung cancer, through simultaneous detection of cytochrome P4501A1 enzyme gene (cytochrome P450, family I, subfamily A, polypeptide 1, CYP1A1), Poly (ADP-ribose) polymerase family member 1 (poly(ADP-ribose) polymerase family, member 1, PARP1, also abbreviated as ADPRT) and excision repair complex 2 (Excision Repair Cross-complementing Rodent Repair Deficiency, Complementation Group 2, ERCC2 ) single nucleotide polymorphism (SNP) to predict an individual's susceptibility to lung cancer. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from the bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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