Milk containing thymus polypeptide extraction and preparation method thereof
A technology of thymus polypeptide and extract, which is applied in the field of milk containing thymus polypeptide extract and its preparation process, can solve problems such as no research reports, achieve good economic and social benefits, stimulate stem cell proliferation, and enhance immunity Effect
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Embodiment 1
[0033] Collect fetal bovine thymus tissue; wash the fresh fetal bovine thymus tissue with ultrapure water at room temperature; add 4 times the volume of sterile ultrapure water to make a homogenate; add 5 times the volume of sterile ultrapure water, mix Homogenize, freeze and thaw twice at -20°C; centrifuge the freeze-thawed homogenate at 4-8°C at 8,000 rpm for 20 minutes to remove the precipitate; trap the supernatant with a tangential flow ultrafiltration device, Thymus polypeptide extract with a molecular weight of less than 30,000 Daltons; the content of polypeptides with a molecular weight lower than 90 Daltons in the thymus polypeptide extract is 49.4% to 58.3%, and the content of polypeptides with a molecular weight of 90 to 130 Daltons is 10.6% to 12.9%. The peptide content of 130-270 Daltons is 21.7%-27.1%, and the content of peptides of 270-30000 Daltons is 7.1-12.9%. Sterilize and filter to get it; add 1ml thymus polypeptide extract to 200ml fresh milk , packaged, r...
Embodiment 2
[0035] Collect fetal bovine thymus tissue; wash the frozen fetal bovine thymus tissue with ultrapure water at room temperature; add 5 times the volume of sterile ultrapure water to make a homogenate; add 4 volumes of sterile ultrapure water and mix well , placed at -15°C and repeated freezing and thawing for 3 times; centrifuged the frozen-thawed homogenate at a temperature of 4-8°C at 8,000 rpm for 20 minutes to remove the precipitate; intercepted the supernatant with a tangential flow ultrafiltration device, and the molecular weight was < 30,000 daltons of thymus polypeptide extract; the content of polypeptides with a molecular weight lower than 90 daltons in the thymus polypeptide extract is 49.4% to 58.3%, the content of polypeptides with a molecular weight of 90 to 130 daltons is 10.6% to 12.9%, and the content of polypeptides with a molecular weight of 130 to 130 daltons The peptide content of 270 Daltons is 21.7% to 27.1%, and the content of peptides of 270 to 30,000 Dal...
Embodiment 3
[0037] Collect fetal bovine thymus tissue; wash fresh fetal bovine thymus tissue with ultrapure water at room temperature; add 3 times the volume of sterile ultrapure water to make a homogenate; add 7 volumes of sterile ultrapure water and mix well , placed at -25°C and repeated freezing and thawing twice; the frozen-thawed homogenate was centrifuged at 4-8°C at 8,000 rpm for 20 minutes to remove the precipitate; the supernatant was intercepted with a tangential flow ultrafiltration device, and the molecular weight was < 30,000 daltons of thymus polypeptide extract; the content of polypeptides with a molecular weight lower than 90 daltons in the thymus polypeptide extract is 49.4% to 58.3%, the content of polypeptides with a molecular weight of 90 to 130 daltons is 10.6% to 12.9%, and the content of polypeptides with a molecular weight of 130 to 130 daltons The peptide content of 270 Daltons is 21.7% to 27.1%, and the content of peptides of 270 to 30,000 Daltons is 7.1 to 12.9%...
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