Molecule mark linked to cabbage green bolting gene and establishing method thereof

Abstract

The invention discloses a method for establishing molecule markings which are linked with immature bolting genes of cabbages. The invention overcomes the defects of large work load, easiness of beingaffected by environment, long breeding cycle and so on of a general marking method. By adoption of RAPD molecule marking method, filtration of immature bolting gene markings is performed on immature bolting amiable cabbage parent strains, immature bolting resistant cabbage parent strains and filial generations '2002-45x2002-90'of the parent strains. PCR reaction adopts 10bp oligonucleotide randomprimers synthesized by Shanghai Sangon Biological Engineering Co., Ltd, and molecule markings S97<392> and U16<446> which are linked with the immature bolting genes of the cabbages are obtained afterfiltration. The markings can be used for early evaluation of immature bolting of the cabbages and molecule marking auxiliary breeding aiming at the characteristic. The invention is of great value in cabbage breeding practice and immature bolting theoretical research.

Description

technical field [0001] The invention relates to a method for establishing a molecular marker, in particular to a method for establishing a molecular marker linked with the immature bolting gene of cabbage. Background technique [0002] Cabbage (Brassica Oleracea L.Var.Capitata L.) is a biennial vegetable crop of Brassica genus Brassica, which is widely distributed in my country. In recent years, the planting area of ​​spring cabbage has increased rapidly, but due to the poor tolerance of immature bolting of some spring cabbage varieties planted, coupled with the frequent occurrence of late spring and cold weather in spring and long-term cloudy and less sunny weather, the cultivated spring cabbage varieties often occur underripe Bolting phenomenon seriously affects the quality and yield of cabbage. According to the survey, the unripe bolting rate of spring cabbage in the southern region was always between 15% and 50% between 1998 and 2005, and some farmers sometimes reached ...

AI Technical Summary

Problems solved by technology

[0004] However, at present, the breeding of new varieties of cabbage resistant to immature bolting is based on traditional breeding methods, and the selection and identification of immature bolting resistance traits need to be carried out across the year, which takes a long period and a large workload

In addition, the trait of immature bolting resistance is greatly affected by genetic background and environmental factors, which makes it difficult to rely on phenotypic selection.

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