Unlock instant, AI-driven research and patent intelligence for your innovation.
Kit for estimating pre-pregnant nourishment metabolism inheritance capability
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of nutrient metabolism and kit, applied in fields involving molecular biology and medicine
Inactive Publication Date: 2008-08-13
HAINAN ZHUJIAN BIOTECH
View PDF0 Cites 19 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Polymorphisms in this gene cause rickets caused by vitamin D deficiency
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
[0036] Use a real-time quantitative PCR detection kit for evaluating the genetic ability of nutritional metabolism before pregnancy, which contains the following primer pairs and fluorescent probe pairs:
[0037] Sense primer 1: 5'-AAAGCTGCGTGA-3' (SEQ ID NO: 1)
[0038] Antisense primer 1: 5'-TGAAGGAGAAGGTG-3' (SEQ ID NO: 2)
[0039] Sense primer 2: 5'-AAGAACGAAGACTTCA-3' (SEQ ID NO: 3)
[0040] Antisense primer 2: 5'-GGGAGGAGCTGAC-3' (SEQ ID NO: 4)
[0041] Sense primer 3: 5'-GCCATCGCAGAA-3' (SEQ ID NO: 5)
[0042] Antisense primer 3: 5'-ATCCATGTACCACAG-3' (SEQ ID NO: 6)
[0043] Sense primer 4: 5'-TGGACAGGCGGTCC-3' (SEQ ID NO: 7)
[0044] Antisense primer 4: 5'-CTGGGGTGCAGGAC-3' (SEQ ID NO: 8)
[0045] Sense primer 5: 5'-GAGCAGAGCCTGAGTATT-3' (...
Embodiment 2
[0071] Example 2. Services to assess pre-pregnancy genetic capacity for nutritional metabolism
[0073] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica geladsorption method was used to extract DNA from oral epithelial cells.
[0075] Using the kit provided by the invention, the rs1801133 and rs1801131 SNP sites on the 5,10-methylenetetrahydrofolate reductase gene and the rs1801394 methionine synthetase reductase gene on the genomic DNA of the subject were detected SNP sites, rs731236 and rs1544410 on the vitamin D receptor gene, and rs5443 on the G protein β3 subunit gene were detected by fluorescent quantitative PCR to determine the genotypes of these six SNPs.
[0076] Step 3: Analysis of genetic capacity for nutritional metabolism before pregnancy
[0077] Through the analysis of the SNPs genotype of the...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses an agent box for assessing pre-pregnant nutritionmetabolism hereditary ability. The agent box comprises specificity primer pair and specificity fluorescent detecting probe pair for detecting synchronously number rs1801133 and rs1801131 SNP site on 5,10-methano tetrahydrofolic acid reducing ferment gene, number rs1801394 SNP site on methilanin synthetase reducing ferment gene, number rs731236 and rs1544410 on vitamin D receptorgene, number rs5443 SNP site on G albumen Beta3 second unit gene, general component for detecting fluorescent definite quantity PCR etc.. The agent box of the invention assesses pre-pregnant nutritionmetabolism hereditary ability by detecting synchronously mononucleotide polymorphism site gene type correlative closely to pre-pregnant nutritionmetabolism hereditary potence.
Description
technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for assessing the genetic ability of pre-pregnancy nutritional metabolism, by simultaneously detecting 5,10-methylene tetrahydro Single nucleotide polymorphism locus genes of folate reductase gene (MTHFR), methionine synthase reductase gene (MTRR), vitamin D receptor gene (VDR), G protein β3 subunit gene (GNB3) type to assess pre-pregnancy nutritional metabolism genetic ability. Background technique [0002] Nutrition is the material basis of human health. Pre-pregnancy / perinatal nutrition is particularly important because it not only affects the health of pregnant women, but also affects the normal development of the fetus and the physical and mental status of infants and young children after birth. Surveys have shown that the lack of protein before pregnancy and during pregnancy is not conducive to the development...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
