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Kit for estimating pre-pregnant nourishment metabolism inheritance capability

A technology of nutrient metabolism and kit, applied in fields involving molecular biology and medicine

Inactive Publication Date: 2008-08-13
HAINAN ZHUJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polymorphisms in this gene cause rickets caused by vitamin D deficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Use of detection kits

[0033] Step 1: Extraction of DNA template

[0034] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0035] Step 2: Real-time quantitative PCR reaction

[0036] Use a real-time quantitative PCR detection kit for evaluating the genetic ability of nutritional metabolism before pregnancy, which contains the following primer pairs and fluorescent probe pairs:

[0037] Sense primer 1: 5'-AAAGCTGCGTGA-3' (SEQ ID NO: 1)

[0038] Antisense primer 1: 5'-TGAAGGAGAAGGTG-3' (SEQ ID NO: 2)

[0039] Sense primer 2: 5'-AAGAACGAAGACTTCA-3' (SEQ ID NO: 3)

[0040] Antisense primer 2: 5'-GGGAGGAGCTGAC-3' (SEQ ID NO: 4)

[0041] Sense primer 3: 5'-GCCATCGCAGAA-3' (SEQ ID NO: 5)

[0042] Antisense primer 3: 5'-ATCCATGTACCACAG-3' (SEQ ID NO: 6)

[0043] Sense primer 4: 5'-TGGACAGGCGGTCC-3' (SEQ ID NO: 7)

[0044] Antisense primer 4: 5'-CTGGGGTGCAGGAC-3' (SEQ ID NO: 8)

[0045] Sense primer 5: 5'-GAGCAGAGCCTGAGTATT-3' (...

Embodiment 2

[0071] Example 2. Services to assess pre-pregnancy genetic capacity for nutritional metabolism

[0072] Step 1: DNA Extraction

[0073] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0074] Step 2: Genotyping Assays

[0075] Using the kit provided by the invention, the rs1801133 and rs1801131 SNP sites on the 5,10-methylenetetrahydrofolate reductase gene and the rs1801394 methionine synthetase reductase gene on the genomic DNA of the subject were detected SNP sites, rs731236 and rs1544410 on the vitamin D receptor gene, and rs5443 on the G protein β3 subunit gene were detected by fluorescent quantitative PCR to determine the genotypes of these six SNPs.

[0076] Step 3: Analysis of genetic capacity for nutritional metabolism before pregnancy

[0077] Through the analysis of the SNPs genotype of the...

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PUM

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Abstract

The invention discloses an agent box for assessing pre-pregnant nutrition metabolism hereditary ability. The agent box comprises specificity primer pair and specificity fluorescent detecting probe pair for detecting synchronously number rs1801133 and rs1801131 SNP site on 5,10-methano tetrahydrofolic acid reducing ferment gene, number rs1801394 SNP site on methilanin synthetase reducing ferment gene, number rs731236 and rs1544410 on vitamin D receptor gene, number rs5443 SNP site on G albumen Beta3 second unit gene, general component for detecting fluorescent definite quantity PCR etc.. The agent box of the invention assesses pre-pregnant nutrition metabolism hereditary ability by detecting synchronously mononucleotide polymorphism site gene type correlative closely to pre-pregnant nutrition metabolism hereditary potence.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for assessing the genetic ability of pre-pregnancy nutritional metabolism, by simultaneously detecting 5,10-methylene tetrahydro Single nucleotide polymorphism locus genes of folate reductase gene (MTHFR), methionine synthase reductase gene (MTRR), vitamin D receptor gene (VDR), G protein β3 subunit gene (GNB3) type to assess pre-pregnancy nutritional metabolism genetic ability. Background technique [0002] Nutrition is the material basis of human health. Pre-pregnancy / perinatal nutrition is particularly important because it not only affects the health of pregnant women, but also affects the normal development of the fetus and the physical and mental status of infants and young children after birth. Surveys have shown that the lack of protein before pregnancy and during pregnancy is not conducive to the development...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11G01N21/64
Inventor 冯哲民邹祖烨
Owner HAINAN ZHUJIAN BIOTECH
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