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Bacteriostasis composition for garbage burying field and preparation thereof

A technology for landfills and compositions, applied in the directions of botanical equipment and methods, chemicals for biological control, applications, etc., can solve the problems of environmental pollution, small investment, flies infestation, etc., and achieve significant social benefits , the effect of inhibiting the growth of flies and the reproduction of harmful microorganisms

Inactive Publication Date: 2008-09-24
戴志南
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current disposal methods of garbage generally include landfill, incineration, etc. Landfill is a relatively commonly used method with small investment and convenient operation, but there are also many defects, especially the larvae and larvae of flies in the garbage. Maggot pupae, if not pre-treated, will directly form secondary pollution, such as garbage dumps, often plagued by flies, directly affecting the surrounding environment
At the same time, if the harmful microorganisms in the garbage are not suppressed, it will also lead to serious pollution of the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Inoculate yeast, lactic acid bacteria and Bacillus subtilis strains in respective activation culture medium, activate culture, culture time is 3 days, culture temperature is 35 ℃;

[0053] (2) Then the mixture of each activated bacterial classification and the activated medium is mixed and inoculated in the mother liquor culture medium, and the mother liquor anaerobic culture is carried out under airtight, the culture time is 15 days, and the culture temperature is 40 DEG C;

[0054] The ratio of the mixing amount of each strain is:

[0055] The total number of yeast colonies: the total number of lactic acid bacteria colonies: the total number of Bacillus subtilis colonies = 2 × 10 3 cfu: 3×10 5 cfu: 2×10 3 cfu;

[0056] The total addition of the mixture of the activated bacterial classification and the activation medium is 10% of the weight of the mother liquor culture medium;

[0057] (3) 3% of the mother liquor of step (2) is added in the fermentation medium ...

Embodiment 2

[0085] Toxicity test adopts the animal test method stipulated in GB15670-1995 to test the product of embodiment 1, and the results are as follows:

[0086] Acute oral LD ​​in male and female mice 50 Values ​​are greater than 5000mg / kg.b.wt.

Embodiment 3

[0088] Antibacterial test:

[0089] The bacteriostatic composition that is used for landfill that embodiment 1 obtains is diluted with water, is formulated as the aqueous solution that content is 50mg / ml, with 1 kilogram / meter 2 The amount of spraying on the rubbish surface, using the (2001) quantity recognition (Su) word (z0188) standard to detect, the Staphylococcus aureus inhibition rate can reach more than 81%, and the fly growth inhibition rate can reach more than 80%.

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Abstract

The invention discloses an antimicrobial compound used in a landfill waste site and a preparation method thereof. The antimicrobial compound includes the components and contents that the sum of a microzyme colony is 1 to 3*10<3>cfu / ml; the sum of a lactobacillus colony is 2 to 4*10<5>cfu / ml; the sum of a Bacillus subtilis colony is 1 to 3*10<3>cfu / ml. The antimicrobial compound used in the landfill waste site of the invention is innoxious and uninjurious, can effectively restrain the growing of flies in garbage and the propagation of spoilage organisms, and has obvious social benefit.

Description

technical field [0001] The invention relates to a strain mixture, in particular to a bacteriostatic composition used in landfills and a preparation method thereof. Background technique [0002] With the continuous improvement of people's living standards and the continuous increase of population, more and more garbage is produced, and the treatment of garbage has attracted people's attention. [0003] The current disposal methods of garbage generally include landfill, incineration, etc. Landfill is a relatively commonly used method with small investment and easy operation. However, there are many defects, especially the larvae and larvae of flies in the garbage. Maggot pupae, if not pre-treated, will directly form secondary pollution, such as garbage dumps, often plagued by flies, directly affecting the surrounding environment. At the same time, if the harmful microorganisms in the garbage are not suppressed, it will also lead to serious pollution of the environment. Cont...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P3/00B09B5/00
Inventor 戴志南
Owner 戴志南
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