Unlock instant, AI-driven research and patent intelligence for your innovation.

Antibody formulations having optimized aggregation and fragmentation profiles

An antibody, preparation technology, applied in the field of antibody preparation with optimized aggregation and fragmentation properties

Inactive Publication Date: 2008-09-24
MEDIMMUNE LLC
View PDF294 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently no treatments to prevent the development of asthma in subjects at higher risk of developing asthma

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antibody formulations having optimized aggregation and fragmentation profiles
  • Antibody formulations having optimized aggregation and fragmentation profiles
  • Antibody formulations having optimized aggregation and fragmentation profiles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0123] 5.1 Methods of preparing antibody preparations

[0124] The present invention provides methods for preparing preparations of antibodies that immunologically specifically bind to an antigen of interest, or derivatives, analogs or fragments thereof. Such antibodies can be purified according to antibody purification methods known in the art. figure 1 with 2 It is another flow chart for preparing purified antibodies. In one embodiment, the method for preparing the liquid formulation of the present invention includes: using a semi-permeable membrane to set a suitable molecular weight (MW) cut-off value (such as intact antibody molecules and F(ab’) 2 Use 30kD cut-off value for fragments; 10kD cut-off value for antibody fragments such as Fab fragments) Concentrate the components containing purified antibodies or fragments to a concentration of about 15 mg / ml, about 20 mg / ml, about 30 mg / ml, and about 40 mg / Ml, about 50mg / ml, about 60mg / ml, about 70mg / ml, about 80mg / ml, about 90m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides methods of optimizing the production and purification of antibody formulations that immunospecifically bind to antigens of interest and are suitable for parenteral administration to a subject, which formulations exhibit increased stability due to reduced degradation and aggregation of the antibody component on long term storage. Such methods provide formulations that offer multiple advantages over formulations produced by non-optimized methods including less stringent or more readily available transportation / storage conditions, and less frequent dosing or smaller dosage amounts in the therapeutic, prophylactic and diagnostic use of such formulations. The invention further provides methods of utilizing the formulations of the present invention.

Description

[0001] According to 35U.S.C.? 19(e), this application claims the priority of the U.S. Provisional Patent Application 60 / 693,603 filed on June 23, 2005 and the U.S. Provisional Patent Application 60 / 699,614 filed on July 15, 2005, the entire contents of which are incorporated herein by reference . [0002] 1 Introduction [0003] The present invention provides methods for optimizing the production and purification of antibody preparations that specifically bind immunologically to subjects of interest and are suitable for parenteral administration. As the degradation and aggregation of antibody components are reduced during long-term storage, the stability of the preparations is improved . Compared with the preparations produced by unoptimized methods, the preparations provided by this method have many advantages, including less stringent transportation / storage conditions or easier accessibility, and the treatment, prevention, and diagnostic applications of such preparations. The fre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10
Inventor 魏紫萍G·I·图斯M·切纳曼C·B·阿伦
Owner MEDIMMUNE LLC