Expressing method of human interleukin 7 in eucaryon host

A technology of interleukin and host, applied in the direction of interleukin, cytokine/lymphokine/interferon, animal/human protein, etc., can solve the problems of reducing protein expression and biological activity, prokaryotic host expression toxicity, etc. , to achieve the effects of easy cultivation and fermentation, favorable biological activity and high yield of target protein
CN101302517BInactive Publication Date: 2010-11-03GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
Publication Date
2010-11-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for expressing human interleukin 7 in eucaryotic host. When the eucaryotic host is pichia X-33, the method comprises the following steps of: (1) cloning an IL-7 gene of human; (2) building an eucaryotic expression vector; (3) transforming recombinant vector to the eucaryotic yeast host; and (4) expressing a rIL-7 protein in the yeast host. A prior method for expressing IL-7 by using prokaryotic host escherichia coli is changed, and a method for expressing IL-7 through eucaryotic host pichia is searched out, thereby ensuring an IL-7 biologic activity and obtaining a large amount of stable protein.
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Description

technical field

[0001] The present invention relates to the technology of applying recombinant DNA technology to produce genetically engineered protein medicine, in particular to a method for expressing human interleukin 7 (rhIL-7) in a eukaryotic host. Background technique

[0002] Human interleukin 7 (human interleukin 7, rhIL-7) is an important member of the cytokine interleukin protein family, which is important for regulating the development and survival of lymphocytes. It was first discovered in 1988, when A.E.Namen et al. isolated this cytokine when studying the development of mouse B cells, and it has the function of stimulating the proliferation of mouse pre-B cells. Subsequent scientific studies have found that the number of T cells and B cells in the mice injected with IL-7 is greatly increased, and the development of the lymphatic system is abnormal in mice knocked out of IL-7 and its receptors, so IL-7 plays a very important role in the development of lymphatic...

Claims

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