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Genetic information and health evaluating management
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A technology of health assessment and genetic risk, applied in the fields of molecular biology and medicine, can solve the problems of inability to determine the real cause of cardiovascular disease, hysteresis, complex mechanism of cardiovascular disease, etc.
Inactive Publication Date: 2009-01-28
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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Abstract
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Problems solved by technology
The above diagnostic measures can diagnose people who have already suffered from cardiovascular diseases, but there is a lag, and patients are often admitted to the hospital for examination after the corresponding clinical symptoms appear
In addition, these diagnostic methods cannot determine the real cause of cardiovascular disease. The mechanism of cardiovascular disease is relatively complicated and requires a scientific and comprehensive explanation.
Method used
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[0041] Step 2: Electrophoretic detection and typing
[0042] Use the electrophoresis detection set in the detection kit, which contains the following primer pairs:
[0043] Sense primer: 5'-CTGGAGACCACTCCCCATCCTTTTCT-3' (SEQ ID NO: 1)
[0044] Antisense primer: 5'-GATGTGGCCATCACATTCGTCAGAT-3' (SEQ ID NO: 2)
[0045] The reaction system has a total volume of 25 μl, including 1 μl of DNA template with a concentration of 12.5ng / μl, 2.5 μl of 10XPCR reaction buffer, 0.2 μl of 25mM dNTP mixture, 1.5 μl of 25mM MgCl2 solution, 0.125 μl of 5units / μl Taq DNA polymerase, 20 μM ACE gene electrophoresis detection primer pair 0.25 μl each, deionized water 19.175 μl.
[0046] The reaction was carried out on a PCR amplification instrument, and the reaction conditions were 94° C. for 12 minutes, and 30 cycles of 94° C...
Embodiment 2
[0071] Example 2. Services for Individual Cardiovascular Health Assessment and Management of People
[0073] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica geladsorption method was used to extract DNA from oral epithelial cells.
[0075] Using the kit provided by the present invention, carry out PCR amplification and electrophoresis detection on the SNP site No. rs4646994 on the ACE gene of the subject's genomic DNA, and perform SNP site No. rs5186 on the AGTR1 gene, SNP site No. rs5443 on the GNB3 gene, The SNP site rs1799983 on the NOS3 gene was detected by fluorescent quantitative PCR to determine the genotypes of the four SNP sites.
[0076] Step 3: Analysis of Individual Genetic Risk of Cardiovascular Disease
[0077] Through the analysis of the SNP genotype of the tested subjects, an analysis report ...
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Abstract
The invention discloses a kit that is used for genetic risk detection of individual angiocardiopathy as well as health evaluation and management. The kit comprises an electrophoresis detecting primer pair that is used for detecting rs4646994 SNP polymorphism genotype on the gene of angiotonin converting enzyme (ACE), particularity primer pairs and particularity fluorescent probe pairs that are used for detecting rs5186 SNP locus on the gene of angiotonin II-1 type receptor (AGTR1), rs5443 SNP locus on the gene of G protein 3 subunit (GNB3) and rs1799983 SNP locus on the gene of endothelial nitric oxide synthase (NOS3), a fluorescent quantitative PCR routine component, and a PCR reaction component, etc. The kit of the invention implements cardiovascular health evaluation of detected person by evaluating the generic risk of developing angiocardiopathy and makes a health management plan that satisfies the genetic predisposition of detected person according to the four SNP locus genotypes that related with the genetic risk of developing angiocardiopathy of the detected person.
Description
technical field [0001] The invention relates to the fields of molecular biology and medicine, and more specifically, the invention relates to a kit for detecting individual genetic information for health assessment and management. Background technique [0002] Cardiovascular disease kills 12 million people every year, which is close to 1 / 4 of the world's total death toll, and has become the number one enemy of human health. Although in the past 30 years, the mortality rate of cardiovascular disease has declined to varying degrees in most countries except Eastern European countries, it still ranks first among the causes of death among men over 45 years old in most countries, and is second only to tumors among women. The second cause of death seriously affects human life expectancy and quality of life. As my country enters an aging society, cardiovascular and cerebrovascular diseases are serious problems facing the whole society. [0003] Medical experts generally agree that...
Claims
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IPC IPC(8): G01N21/00G01N33/50C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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