Rat model of intrinsic heat blood stasis syndrome

A technology of rat model and blood stasis syndrome, which is applied in the establishment of rat models of heat accumulation and blood stasis syndrome, and in the field of rat model establishment, can solve the problems of short survival time, poor model stability, and ununiform detection indicators, etc., and achieve Good stability and long survival time

Inactive Publication Date: 2009-02-18
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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Problems solved by technology

However, the above-mentioned animal models of heat accumulation and blood stasis syndrome have poor stability; the survival time is very short, generally no more than 24 hours; there is still a gap between the simulated symptoms and the actual clinical manifestations of traditional Chinese medicine; the objective detection indicators are not uniform enough
Therefore, using the models reported in the past to analyze the biological basis of "heat accumulation and blood stasis syndrome" seems to lack sufficient scientific basis

Method used

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  • Rat model of intrinsic heat blood stasis syndrome
  • Rat model of intrinsic heat blood stasis syndrome
  • Rat model of intrinsic heat blood stasis syndrome

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0041] 1. Production materials

[0042] 1 Experimental animals:

[0043]Adult male SD rats (Sprague-Dawley rats), weighing 190-210 g, SPF grade, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., animal license number: SCXK (Beijing 2002-0003).

[0044] 2 main reagents:

[0045] 2.5mg / mlLPS (Escherichia coli 055:B5, Sigma company)

[0046] 2. Production of Model Rats with Heat Accumulation and Blood Stasis Syndrome

[0047] 80 SD rats were given free access to food and water, and the animals were divided into model group (n=48) and normal control group (n=32) according to the random number table method. 8 at each time point. Before the injection, the rats were weighed, and the experimental group was intraperitoneally injected with LPS at a dose of 2.5 mg / Kg, and the injection concentration of LPS was 2.5 mg / ml; the control group was injected with normal saline 2.5 mg / Kg. 2 injections per week, one on Monday and one on Thursday. The four e...

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Abstract

The invention relates to a model of a rat suffered from heat blood stasis syndrome, which makes the induction by continuous LPS abdominal cavity injection of the rat. The model is characterized in that the continuous LPS abdominal cavity injection is made twice a week, namely one injection at intervals of three days or four days. The rat model makes the induction by continuous LPS abdominal cavity injection of the rat, so that the established heat blood stasis syndrome animal model is good in stability and long in survival time, and the simulated symptoms are nearly line with the actual clinical performance and the objective detection indexes, not only the model is more similar to the presentation of the blood stasis syndrome in extrinsic feature, but also the mode can be judged by utilizing a plurality of more detailed biological indicators.

Description

technical field [0001] The invention relates to a method for establishing a rat model, in particular to a method for establishing a rat model of heat accumulation and blood stasis syndrome and its identification, and belongs to the field of basic research and clinical application of blood stasis syndrome in traditional Chinese medicine. Background technique [0002] Cardiovascular and cerebrovascular diseases are still a complex and major disease that seriously endangers the health of middle-aged and elderly people in my country, and it is also a difficult problem in the world's medical field. Blood stasis syndrome is a common basic syndrome in TCM clinical practice, and it is the pathophysiological basis of coronary heart disease, diabetes, hypertension and cerebrovascular disease. Taking blood stasis syndrome as the entry point to study the essence of syndrome has a profound theoretical basis and rich clinical practice. On the one hand, the common symptoms and signs of bl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A01K67/027
Inventor 徐群渊周岩江欢杨超孙晓红李林田金洲
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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