Potsdam Bacillus brevis and use thereof

A technology of Bacillus brevius and bacterial agents, applied in the direction of bacteria, mining fluids, microorganisms, etc., can solve problems such as lack of research rules and methods that can be followed

Active Publication Date: 2009-04-15
DAQING OILFIELD CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Analyzing research data at home and abroad, it is found that microbial oil recovery technology has been developed for more than half a century since 1926. However, the use of microbial / chemical flooding to further enhance oil recovery after polymer flooding is a relatively cutting-edge technology. It is still a field experiment, just a preliminary exploration, and there are no research rules and methods to follow

Method used

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  • Potsdam Bacillus brevis and use thereof
  • Potsdam Bacillus brevis and use thereof
  • Potsdam Bacillus brevis and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Screening, cultivation and storage of Brevibacillus borstelensis Po CGMCC No.2441.

[0039] Bacteria enrichment medium (g / L, medium compounded with inorganic salts): inorganic salts (KH 2 PO 4 0.5-1.0%, K 2 HPO 4 0.75-1.5%, Na 2 HPO 4 0.1-0.5%, (NH 4 ) 2 SO 4 0.1-0.5%, CaCl 2 0.001% and FeSO 4 0.001%, crude oil 1-10%; polymer (molecular weight 15 million) 0.05-0.1%, pH 6.8-7.5, 121°C, sterilized for 15-20min. Cultivate in a shaker at 45°C and 120rpm for 5-7 days.

[0040] Oil plate medium (g / L): KH 2 PO 4 0.5-1.0%, K 2 HPO 4 0.75-1.5%, Na 2 HPO 4 0.1-0.5%, (NH 4 ) 2 SO 4 0.1-0.5%, CaCl 2 0.001% and FeSO 4 0.001%, crude oil 1-10%, polymer (molecular weight: 15 million) 0.05-0.1%, agar 1.5-2.0%, pH6.8-7.5, 121°C, sterilized for 15-20min.

[0041] Incline medium (g / L): peptone 10, beef extract 3, sodium chloride 5, water 1000mL, pH7.0-7.2.

[0042] The bacterial strains to be screened must not only adapt to the environmental con...

Embodiment 2

[0048] Embodiment 2, the performance evaluation of Potsdam brevibacillus (Brevibacillus borstelensis) Po CGMCC No.2441

[0049] The performance of the Potsdam brevibacillus (Brevibacillus borstelensis) Po CGMCC No.2441 that embodiment 1 obtains is evaluated from the following aspects:

[0050] 1. Determination of surface activity of strain fermentation broth

[0051] Measure the oil discharge activity of the biosurfactant produced by Potsdam Brevibacillus borstelensis (Brevibacillus borstelensis) Po CGMCCNo.2441 with the following method: get a petri dish, add water, add 0.1-0.2mL n-alkane on the water surface to form an oil film (at 45 ℃), and then add the fermented liquid obtained in Step 2 of Example 1 to the center of the oil film. As a result, the center oil film is squeezed to form a circle around, and the diameter of the circle is directly proportional to the content and activity of the surfactant, and the diameter of the circle produced by the action of the strain is ...

Embodiment 3

[0058] Embodiment 3, the effect of Potsdam brevibacillus (Brevibacillus borstelensis) Po CGMCC No.2441 on crude oil alkane composition

[0059] Detect the effect of Brevibacillus borstelensis Po CGMCC No.2441 on the alkane composition of crude oil, method: first take a 250mL triangular flask, add 5-10g of oil sample, Brevibacillus borstelensis Po CGMCC No.2441 bacterial liquid 10mL (bacteria content> 10 7 cfu / mL) and enriched culture medium 150mL, shake culture at 45-50°C and 150rpm for 7-15 days. Standard SY / T 5779-1995 Crude Oil Full Hydrocarbon Gas Chromatographic Analysis Method) Perform full hydrocarbon chromatographic analysis of crude oil before and after the treatment of Brevibacillus borstelensis Po CGMCC No.2441 (parallel detection 3 times).

[0060] The result is as Figure 1B As shown in Table 3, it can be seen from the parameters of the whole hydrocarbon chromatographic analysis of crude oil before and after the action of Potsdam Brevibacillus borstelensis (Brev...

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Abstract

The invention discloses a Brevibacillus borstelensis Po CGMCC No. of 2441 and the application thereof. A microbial inoculum that contains the brevibacillus borstelensis Po CGMCC No. of 2441 also belongs to the extent of protection of the invention. Model oil displacing experiment results indicate that after the oil displacement with polymers, the brevibacillus borstelensis oil displacement can be used for raising the recovery factor by 3 to 5 percent (OOIP), the brevibacillus borstelensis oil displacement combined with a polymer protecting slug can be used for raising the recovery factor by about 7 percent (OOIP), and a brevibacillus borstelensis oil displacing method that is combined with chemical oil displacement can be used for raising the recovery factor by 6 to 13 percent (OOIP). The experiment results indicate that the strain can be widely applied into the field of oil exploiting industries and particularly into the field of enhanced microorganism oil exploiting, and is applicable to large-area popularization and application.

Description

technical field [0001] The invention relates to the field of microbial oil recovery, in particular to a strain of Brevibacillus borstelensis Po and its application in oil recovery engineering. Background technique [0002] Petroleum is a non-renewable energy source. In order to effectively utilize petroleum resources, countries all over the world are looking for efficient methods that can enhance the recovery rate, so as to exploit the crude oil that remains in the formation and is difficult to recover by conventional oil recovery methods. Since the 1980s, many countries have carried out research on the technology of enhancing oil recovery by using microorganisms. After more than 20 years of hard work, this technology has made great progress. [0003] Polymer flooding in Daqing Oilfield has made breakthroughs through key scientific and technological breakthroughs in the eighth and ninth five-year plans of the country. It has entered the stage of industrial application from p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20E21B43/22C12R1/01
Inventor 石梅李蔚侯兆伟陈星宏郭盟华
Owner DAQING OILFIELD CO LTD
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