Method for separation and purification of human amniotic fluid stem cell

A technology for separation and purification of stem cells, applied in the field of cell separation and purification, which can solve the problems affecting the efficiency of multidirectional induction and differentiation of cells, and the difficulty in obtaining biological traits of hAFSCs, etc., to achieve good proliferation and induction of differentiation, and the effect of uniform traits
CN101418284AInactive Publication Date: 2009-04-29FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
Publication Date
2009-04-29
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention discloses a method for separating and purifying human amniotic fluid stem cells. The method is to use an embryo antigen-4 at a special stage as a screening marker to separate and purify the human amniotic fluid stem cells which have homogeneous characteristics and are positively expressed by the embryo antigen-4 at the special stage from the human amniotic fluid stem cells which are adherently cultured. Experimental results show that the method can obtain the human amniotic fluid stem cells which have homogeneous characteristics, good capacities of proliferation and inductive differentiation, and cell ratio of G0 stage to G1 stage cells of more than 90 percent, and can express specificity markers of human totipotent stem cells such as the embryo antigen-4, Nanog and Oct-4 at the special stage. The invention provides a new way to obtain the human amniotic fluid stem cells, and lays a foundation for the further application of the human amniotic fluid stem cells.
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Description

technical field

[0001] The invention relates to a method for separating and purifying cells, in particular to a method for separating and purifying human amniotic fluid stem cells. Background technique

[0002] The research on human amniotic fluid-derived stem cells (hAFSCs) has gradually attracted attention in recent years. In 2003, In't Anker et al. confirmed that hAFSCs could be isolated and cultured from amniotic fluid in the second trimester (17-22 weeks), which opened up a new way to obtain adult stem cells, which aroused great interest and attention of researchers. Current research shows that hAFSCs not only have the biological characteristics of general adult stem cells, but also have good proliferation ability and multi-lineage differentiation potential, and can also express some marker molecules unique to totipotent stem cells such as SSEA-4 (stage-specific embyronic antigen-4). 4. Special stage embryonic antigen-4), Oct-4 and nanog, etc., indicating that hAFSCs a...

Claims

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