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A kind of sulfated Grifola frondosa water-insoluble polysaccharide and its application

A technology of water-insoluble polysaccharide and Grifola frondosa polysaccharide, which is applied in directions such as allergic diseases and drug combinations, can solve the problems of undiscovered patent publications and the like, and achieves the effects of enhancing immunity, mild reaction conditions and convenient post-processing

Active Publication Date: 2018-04-10
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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  • A kind of sulfated Grifola frondosa water-insoluble polysaccharide and its application
  • A kind of sulfated Grifola frondosa water-insoluble polysaccharide and its application
  • A kind of sulfated Grifola frondosa water-insoluble polysaccharide and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0047] A kind of sulfated Grifola frondosa water-insoluble polysaccharide, the steps of preparation method are as follows:

[0048] ⑴ Using Grifola frondosa polysaccharide as raw material, swell the Grifola frondosa polysaccharide with N,N-dimethylformamide, the ratio mg:mL of the Grifola frondosa polysaccharide to N,N-dimethylformamide is 200- 300: 1-5mL, then add sulfur trioxide trimethylamine salt [(CH 3 ) 3 N·SO 3 ], the mass ratio of the Grifola frondosa polysaccharide to trimethylamine sulfur trioxide is 400-600:1-4, stirred and reacted in a water bath at 50-90°C for 5-7h to obtain a reaction product;

[0049] (2) Cool the reaction product to room temperature, centrifuge at 3000-5000r / min to discard the supernatant, add absolute ethanol to the precipitate, centrifuge at 3000-5000r / min for 8-15min, and wash the polysaccharide 3 times with ethanol until the sulfur trioxide trimethylamine salt is dissolved. The fishy smell is eliminated, and there are many sugar deposits...

Embodiment 2

[0072] A kind of sulfated Grifola frondosa water-insoluble polysaccharide, the steps of preparation method are as follows:

[0073] (1) Using Grifola frondosa polysaccharide as raw material, swell 400-600mg of Grifola frondosa polysaccharide with 2-10mL N,N-dimethylformamide, add 1-4g sulfur trioxide trimethylamine salt, and bathe in water at 50-90°C Stir the reaction for 5-7h;

[0074] (2) Cool the reaction product to room temperature, centrifuge at 3000-5000r / min to discard the supernatant, precipitate and add absolute ethanol, centrifuge at 3000-5000r / min for 8-15min, and wash the polysaccharide with ethanol for 3 times until the fishy smell of trimethylamine odor elimination;

[0075] (3) Dissolve the obtained polysaccharide precipitate in 30-70ml distilled water, adjust the pH to 7 with NaOH solution, and centrifuge at 4000r / min for 20min;

[0076](4) Pack the supernatant obtained after centrifugation into a dialysis bag, run water dialysis for 1-3d, distilled water dia...

Embodiment 3

[0089] A kind of sulfated Grifola frondosa water-insoluble polysaccharide, the steps of preparation method are as follows:

[0090] Swell 500 mg of GFP (Grifola frondosa polysaccharide) with 6 mL of DMF, add 2.5 g of trimethylamine sulfur trioxide [(CH3)3N·SO 3 ], and stir in a water bath at 80°C for 6 hours. Cool the reaction product to room temperature, discard the supernatant by centrifugation, add absolute ethanol to the precipitate, centrifuge at 4000r / min for 10min, and wash the polysaccharide with ethanol centrifugation repeatedly for 3 times until the fishy smell of trimethylamine is eliminated. The obtained polysaccharide precipitate was dissolved in 50 ml of distilled water, adjusted to pH 7 with NaOH solution, and centrifuged at 4000 r / min for 20 min. Put the supernatant obtained after centrifugation into a dialysis bag (COMW7000), dialyze with running water for 2 days, and dialyze with distilled water for 1 day. Add ethanol to the dialysate in the bag, and the etha...

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Abstract

The invention relates to a sulfated Grifola frondosa water-insoluble polysaccharide. The preparation method comprises the following steps: using Grifola frondosa polysaccharide as a raw material, swelling with N,N-dimethylformamide, adding sulfur trioxide and trimethylamine salt, and passing through a water bath Reaction, cooling, centrifugation, washing, dialysis in the system. Within a certain concentration and time range of this polysaccharide, S-GFP can significantly improve the proliferation ability and phagocytic activity of RAW264.7 cells in a dose-dependent manner. Activation of RAW264.7 cells shows obvious activation characteristics, and at the same time enhances the metabolism of nucleic acid and glycogen in cells, significantly improves the secretion and activity of acid phosphatase, lysozyme and superoxide dismutase; can induce up-regulation at the mRNA transcription level The expression of iNOS gene in RAW264.7 cells can increase the production of cellular NO, and at the same time promote the secretion of cytokines such as IL-2, IL-12, IFN-γ, IL-6, etc. It has high immune enhancement activity and can be applied to In the preparation of medicines or health products for enhancing immunity.

Description

technical field [0001] The invention belongs to the technical field of food, and relates to a polysaccharide and a preparation method thereof, in particular to a sulfated Grifola frondosa water-insoluble polysaccharide and its application. The sulfated Grifola frondosa water-insoluble polysaccharide can enhance immunity. Background technique [0002] Grifola frondosa (Grifola frondosa), also known as Polyporus frondosa, Thousand Buddha bacteria, etc., is native to Northeast Japan, North America and other places, and is a precious medicinal and edible fungus. Due to the simple breeding method and large yield of Grifola frondosa, it is widely distributed around the world. Among the most important fungi used in the world, the yield of Grifola frondosa ranks second. [0003] Grifola frondosa extract D-fraction has obvious anti-tumor function. This achievement was first reported in the International Medicinal Chemistry Journal "Chemistry and Drug Bulletin" in 1987; The study poi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/04
Inventor 程代王春玲侯丽华陈媛媛韩丽荣孟梦
Owner TIANJIN UNIV OF SCI & TECH
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