Method for preparing deproteinized extract of calf blood and freeze-dried powder thereof

A calf blood, deproteinization technology, applied in freeze-drying delivery, powder delivery, drug combination and other directions, can solve the problems of inactivation of active ingredients, incomplete drying, damage to cell membranes, etc., to improve production efficiency, facilitate industrial production, Avoid the effect of hydrolases

Active Publication Date: 2009-05-20
吉林康乃尔药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent does not give the specific time of pre-freezing and the two drying stages, but we know that for the freeze-drying process, the freeze-drying time is very critical. If the time is too short, the drying will not be complete, resulting in collapsed appearance and bubble pits. If the time is too long, the cell membrane will be damaged and the active ingredients will be inactivated

Method used

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  • Method for preparing deproteinized extract of calf blood and freeze-dried powder thereof
  • Method for preparing deproteinized extract of calf blood and freeze-dried powder thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] calf blood collection

[0040] The blood of calves under 1.5 years old has been tested and stirred with a stirrer at 150 rpm for 20 minutes to remove plasma fibrinogen, then centrifuged with a centrifuge at 800 rpm for 30 minutes, the supernatant was taken and poured into In a clean barrel, freeze at -40°C and then refrigerate at -20°C for later use.

[0041] extract

[0042] Take out the refrigerated calf serum, melt it at room temperature, put it into a vacuum concentration tank, heat to 60°C to coagulate and denature the protein, filter, heat the filtrate to 70°C, concentrate it under reduced pressure to 1 / 4 of the original volume, cool to room temperature, add 5 times the volume of 55% ethanol, ethanol precipitation for 30 hours, filtration, recovery of ethanol to obtain a semi-finished product.

[0043] Decolorization, depyroprotein

[0044] Take the product from the previous step and put it into a hydrolysis tank, add HCl until the pH is 3.5, heat to 70°C, deco...

Embodiment 2

[0048] calf blood collection

[0049] The blood of calf under the age of 1.5 has been tested and stirred with a stirrer at a speed of 100 rpm for 6 minutes to remove plasma fibrinogen, and then centrifuged with a centrifuge at a speed of 2000 rpm for 30 minutes to obtain the supernatant. Pour into a clean bucket, freeze at -40°C and refrigerate at -20°C for later use.

[0050] extract

[0051] Take out the refrigerated calf serum, melt it at room temperature, put it into a vacuum concentration tank, heat to 70°C to coagulate and denature the protein, filter, heat the filtrate to 80°C, concentrate it under reduced pressure to 1 / 8 of the original volume, cool to room temperature, add 2 times 70% ethanol, ethanol precipitation for 24 hours, filtering, recovering ethanol, and obtaining a semi-finished product.

[0052] Decolorization, depyroprotein

[0053] Take the product from the previous step and put it into a hydrolysis tank, add HCl until the pH is 6.5, heat to 60°C, deco...

Embodiment 3

[0057] calf blood collection

[0058] The blood of a calf under 1.5 years old has been tested and stirred with a stirrer at 60 rpm for 12 minutes to remove plasma fibrinogen, and then centrifuged with a centrifuge at 1500 rpm for 30 minutes to take the supernatant , poured into a clean bucket, frozen at -40°C and refrigerated at -20°C for later use.

[0059] extract

[0060] Take out the refrigerated calf serum, melt it at room temperature, put it into a decompression concentration tank, heat to 90°C to coagulate and denature the protein, filter, heat the filtrate to 90°C, concentrate under reduced pressure to 1 / 11 of the original volume, cool to room temperature, add 10 times 94% ethanol, ethanol precipitation for 68 hours, filtering, recovering ethanol, and obtaining a semi-finished product.

[0061] Decolorization, depyroprotein

[0062] Take the product from the previous step and put it into a hydrolysis tank, add HCl until the pH is 5.5, heat to 90°C, decolorize with 1...

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Abstract

The invention aims to provide a method for preparing deproteinized calf blood extract and freeze-dried powder thereof. The method has simple process and is easy for mass production, the obtained extract has high bioactivity and good product purity, the freeze-dried powder is subjected to gradient temperature rise and drying, and the finished product has good appearance and is easy to dissolve. The deproteinized calf blood extract is prepared by the method comprising the following steps: heating collected blood serum and deproteinizing the blood serum, filtering the blood serum and then condensing the filtrate, removing protein by ethanol; then adding active carbon and acid into the filtrate to hydrolyze and decolor the filtrate; filtering the filtrate and then adjusting pH value to a proper range, freezing the filtrate and removing protein repeatedly; and finally using fibrous membrane to hyperfiltrate high molecular substance to obtain the deproteinized calf blood extract, then adding excipient and water for injection into the extract, pre-freezing the extract, and heating the extract in gradient and drying the extract in vacuum to obtain finished products.

Description

technical field [0001] The invention relates to the field of medical biochemical drugs, in particular to a method for preparing calf blood deproteinized extract and freeze-dried powder thereof. Background technique [0002] Calf blood deproteinized extract (serotonin) has been promoted and used in countries all over the world since it was successfully developed by Swiss Sugao Pharmaceutical Factory in 1955 for fifty years. It is a small molecular biologically active substance obtained from calf serum deproteinized from 1 to 18 months. Its injection contains 40±5 mg of total solids of deproteinized calf blood extract per ml, of which 30% are amino acids, Sugars, ketoacids, purines, nucleotides and oligopeptides with a relative molecular weight of 2500-3000, 70% of which are inorganic substances (K + 、Na + , Cl - , Ca 2+ etc.), they can increase the intake and utilization of oxygen by tissue cells, promote the transfer of glucose to brain tissue, and promote the aerobic me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/14A61K9/19A61P1/04A61P1/16A61P9/00A61P9/10A61P9/14A61K35/16
Inventor 宋治国
Owner 吉林康乃尔药业有限公司
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