Suppressor of expression of MCP-1, and ameliorating agent for inflammatory disease, pharmaceutical, supplement, food, beverage or food additive using the suppressor
An inflammatory disease, MCP-1 technology, applied in the field of beverages and food additives, food, can solve the problems of narrow application range, safety problems, difficult long-term intake, etc., and achieve the effect of excellent safety
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[0041] This example is an example of confirming the inhibition of MCP-1 expression by hesperetin at the gene level and protein level.
[0042] (differentiation induction of preadipocytes)
[0043] First, prepare the two types of media shown below, a differentiation-inducing medium and a differentiation-promoting medium.
[0044] differentiation induction medium
[0045] (composition: 0.25μM DEX, 0.5mM MIX, 10μg / mL insulin / DMEM containing 10% FBS)
[0046] 55 mL of FBS (fetal bovine serum (manufactured by GIBCO)) was added to 500 mL of DMEM (manufactured by SIGMA) to prepare 10% FBS-containing DMEM. 138.75 µL of DMS O (manufactured by Nacalai tesque) containing 1 mM DEX (dexamethasone) and 555 µL of PBS (manufactured by SIGMA) containing 10 mg / mL insulin were added thereto. In the insulin-containing PBS, 1N HCl was added to PBS in advance to make it acidic enough to dissolve insulin, and then the insulin was dissolved. Before use, MIX (3-isobutyl-1-methylxanthine) (manufa...
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