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Ammonia ion diagnosis/measuring reagent kit and ammonia ion concentration determination method

A kit and ion technology, applied in the field of ammonia concentration determination, ammonia diagnosis/measurement kit, can solve the problem of undiscovered blood ammonia determination method, etc.

Inactive Publication Date: 2009-06-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching Chinese patents, only found 87105593.7 patent application which discloses a quick-frozen cup for measuring blood ammonia, but did not find an ideal method for measuring blood ammonia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0042] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0043] Stabilizer 500mmol / L

[0044] Coenzyme 3mmol / L

[0045] Glutamine Synthetase 10000U / L

[0046] Glyceraldehyde-3-phosphate dehydrogenase 12000U / L

[0047] Glutamic acid 8mmol / L

[0048] Adenosine triphosphate 3mmol / L

[0049] Glyceraldehyde-3-phosphate 5mmol / L

[0050] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0051] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the ammonia (ion) sample to the reagent is 1 / 25. The reaction direction is positive reaction (rising reaction), the delay time i...

Embodiment 2

[0054] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0055] Reagent 1

[0056] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0057] Stabilizer 50mmol / L

[0058] Coenzyme 3mmol / L

[0059]Glutamic acid 8mmol / L

[0060] Adenosine triphosphate 3mmol / L

[0061] Glyceraldehyde-3-phosphate 5mmol / L

[0062] Reagent 2

[0063] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0064] Stabilizer 50mmol / L

[0065] Glutamine Synthetase 10000U / L

[0066] Glyceraldehyde-3-phosphate dehydrogenase 12000U / L

[0067] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0068] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume of the ammonia (ion) sample and reage...

Embodiment 3

[0071] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0072] Reagent 1

[0073] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0074] Stabilizer 50mmol / L

[0075] Coenzyme 3mmol / L

[0076] Glutamic acid 8mmol / L

[0077] Adenosine triphosphate 3mmol / L

[0078] Glyceraldehyde-3-phosphate 5mmol / L

[0079] Reagent 2

[0080] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 500mmol / L

[0082] Glyceraldehyde-3-phosphate dehydrogenase 12000U / L

[0083] Reagent 3

[0084] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L

[0085] Stabilizer 500mmol / L

[0086] Glutamine Synthetase 10000U / L

[0087] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0088] When measuring the ammonia (ion) concentration, set it on the automatic biochemical analyzer: t...

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Abstract

The invention relates to a kit for diagnosing / measuring ammonia (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of ammonia (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, coenzyme, glutamic acid, adenyl pyrophosphate, glyceraldehydes-3-phosphoric acid, glutamoyl synthetase, glyceraldehydes-3-phosphate dehydrogenaseand a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of ammonia (ions).

Description

technical field [0001] The invention relates to an ammonia (ion) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring ammonia (ion) concentration, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] Ammonia determination methods include micro-diffusion method, ion exchange method, enzymatic method and ammonia electrode method. The most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode. [0003] The diffusion method is that after the sample is alkalized, NH is released 3 , Titrate the released ammonia with acid, or use the Nessler reaction to form brown-yellow mercuric ammonium iodide for colorimetry. These methods need to be alkalized, and the endogenous ammonia formation will affect the accuracy and precision, so they are rarely used at present; the ion exchange method is more accurate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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