Y chromosome MiniSTR typing reagent kit, preparation and use thereof

A technology of Y chromosome and Y-STR, which is applied in biochemical equipment and methods, DNA/RNA fragments, microbial measurement/inspection, etc. It can solve the problem of poor allele frequency distribution, lower amplification efficiency, and poor gene frequency distribution and other issues, to achieve the effect of wide applicability, high efficiency, and parameter optimization
CN101475994AInactive Publication Date: 2009-07-08CHINA UNIVERSITY OF POLITICAL SCIENCE AND LAW
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA UNIVERSITY OF POLITICAL SCIENCE AND LAW
Publication Date
2009-07-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a Y chromosome parting kit and its preparation method and application. The present invention designs three pairs of public primer pairs, optimizedly select 12 X chromosome STR loci suitable for the genetic distribution of Chinese population and designs MiniSTR primers fro part of loci, and respectively adds the three pairs of public primers to the 5' end of the MiniSTR primers to obtain 24 additional primers; the use of the inventive kit can simultaneously amplify 12 X chromosome STR loci at a single-tube, avoids the competition between these primers and the formation of heterozygosity dimers, increases the amplification efficiency of each locus, wherein, the length of amplification products can be less than the 280bp, and has a high sensitivity (as low as 30pg), a higher STR parting success rate can be obtained and the amplification efficiency of medical examiner DNA degradation samples can be improved. The inventive kit has advantages of high sensitivity, high specificity, high detection efficiency, low preparation cost and the like.
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Description

technical field

[0001] The invention relates to a PCR amplification kit, in particular to a fluorescent multiplex amplification kit for simultaneously detecting 12 Y chromosome MiniSTR loci in a single tube, and also relates to a preparation method of the fluorescent multiplex amplification kit and the The application of the kit in forensic identification belongs to the field of typing and identification. Background technique

[0002] Short tandem repeat (short tandem repeat, STR) is a type of DNA sequence with length polymorphism formed by tandem repetition of 2 to 6 bases as the core unit in the human genome. The number of core units varies and the number of repetitions is different. The genetic polymorphisms that make up the STR. STR is widely distributed and numerous, accounting for about 10% of the human genome. The genetic information contained in it is unmatched by any polymorphic markers in the past. Therefore, STR is currently the most widely used genetic marker fo...

Claims

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