A method for the induction and preparation of allopolyploid among the genus of turnip radish

An allopolyploid, radish technology, applied in the directions of botanical equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problem of double haploid hybrid sterility of turnip radish, inability to carry out genetic and breeding utilization and other problems, to achieve the effect of good technical support, saving manpower and resources, high differentiation and proliferation rate

Active Publication Date: 2020-04-14
JIANGSU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem in the prior art that the double haploid hybrids between the turnip genus are infertile and cannot be used for inheritance and breeding, and to provide a method for inducing and preparing allopolyploid between the turnip radish genus

Method used

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  • A method for the induction and preparation of allopolyploid among the genus of turnip radish
  • A method for the induction and preparation of allopolyploid among the genus of turnip radish
  • A method for the induction and preparation of allopolyploid among the genus of turnip radish

Examples

Experimental program
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Effect test

Embodiment 1

[0037] (1) Get the young shoots of the distant double haploid hybrid asexual seedlings of turnip radish and inoculate them in the medium of MS+1mg / L 6-BA+0.1mg / L NAA+2% sucrose+0.7% agar, Cultivate for 20 days to obtain asexual shoots of distantly related double haploids between the turnip radish genera;

[0038] (2) Inoculate the asexual shoots of the distantly related double haploid between the turnip radish genera through the propagation in 0.02wt% colchicine+MS+1mg / L 6-BA+0.1mg / L NAA+2% sucrose +0.7% agar induction medium for 18 hours to obtain induced sprouts;

[0039] (3) Transfer the induced shoots to the differentiation medium of MS+1mg / L 6-BA+0.5mg / L NAA+2% sucrose+0.7% agar to continue the growth of the seedlings.

[0040] (4) Carry out flow cytometry ploidy analysis to the seedlings, the diploid female parent turnip, the male parent radish of the turnip radish double haploid hybrid, and the turnip radish double haploid hybrid identified by cytology As a control, t...

Embodiment 2

[0042]In step (2), the culture time of the multiplied double haploid asexual shoots on the induction medium is 24 hours, and the rest is the same as in Example 1.

Embodiment 3

[0044] In step (2), the culture time of the multiplied double haploid asexual shoots on the induction medium is 30 h, and the rest are the same as in Example 1.

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Abstract

The invention relates to an induction preparation method for turnip and radish intergeneric allopolyploid. The induction preparation method for turnip and radish intergeneric allopolyploid comprises the following steps: inoculating a bud of a turnip and radish intergeneric distant double haploid hybrid asexual seedling and culturing to obtain a propagation-expanded asexual bud; inoculating the budon an induction culture medium which contains 0.02 wt% of colchicines to obtain an induced bud; transferring the induced bud into a differential medium and carrying out subsequent culturing to obtaina seedling; carrying out analysis of ploidy on tender leaves of the seedling by a flow cytometer; and comparing diploid female parent turnip and male parent radish of turnip and radish double haploidhybrid with turnip and radish double haploid hybrid which has been identified by cytology, taking the product of which the peak value is close to contrast as diploid, taking the product with remarkable single peaks as euploid, and taking the product with two or a plurality of height-similar detached peaks as mixoploid. The method is convenient to operate and high in ploidy identifying efficiency;and the problems that in the prior art, turnip and radish intergeneric double haploid hybrid is infertile, and inheritance and breeding utilization cannot be carried out are solved.

Description

technical field [0001] The invention relates to a method for inducing and preparing allopolyploid among turnip radish genera, belonging to the field of plant polyploid breeding. Background technique [0002] Turnip is a subspecies of Brassica genus Brassica in the family Brassicaceae. Its fleshy root is edible, and its fleshy root is tender and dense. It is used for frying and cooking. It has strong adaptability to the environment and high yield, and is one of the traditional vegetables with a long edible history. Radish is native to my country and is a genus of Brassicaceae. It is eaten with fleshy roots and is one of the main root vegetables. It is rich in nutrition, easy to manage, high in yield, low in cost, long in supply, and resistant to storage and transportation. It has a wide range of uses, not only can be eaten, but also processed, and has a high therapeutic effect. The heterogeneous double haploids created with turnip as the female parent and radish as the male ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H1/08
CPCA01H1/08A01H4/001A01H4/008
Inventor 娄丽娜吕彦迪曾玉兰苏小俊许园园刘哲
Owner JIANGSU ACAD OF AGRI SCI
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