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Triterpene extracts of achyranthes bidentata and uses in osteosporosis resistant medicament

A technology of triterpenoids and extracts, which is applied in the application field of anti-osteoporosis drugs, can solve the problems of anti-osteoporosis that have not been reported, and achieve the effect of small side effects

Inactive Publication Date: 2009-08-19
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing patent applications related to total steroid extract for immunological use, but no report on its anti-osteoporosis

Method used

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  • Triterpene extracts of achyranthes bidentata and uses in osteosporosis resistant medicament
  • Triterpene extracts of achyranthes bidentata and uses in osteosporosis resistant medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The dried root (300g) of Achyranthes bidentata B1. was crushed, heated to reflux with 70%-100% ethanol (600ml), and extracted three times. The extracts were combined and concentrated under reduced pressure to remove the solvent in the extract to obtain ethanol extract (about 79.33 g, 26.44%). Suspend the extract in distilled water (500ml), add n-hexane (500ml x 3 times) for extraction, and combine the extracts to obtain n-hexane extract.

[0017] It is also possible to add petroleum ether (500ml x 3 times) for extraction after n-hexane extraction, and combine the extracts to obtain petroleum ether extract. This extraction can also directly replace n-hexane extraction, and when n-hexane extraction is used, this extraction can also be ignored.

[0018] Then the distilled water suspension of the remaining extract is extracted with ethyl acetate (500ml x 3 times), n-butanol (500ml x 3 times) and water respectively to obtain ethyl acetate and n-butanol extract. Extraction ...

Embodiment 2

[0026] The n-hexane extract that embodiment 1 obtains is made into 3 kinds of concentrations described in the following table with Ham's F-12 culture fluid. Use suckling mice 2 days after birth, subcutaneously inject 45CaCl2 (2?Ci), 2 days later, remove the skull, use Ham's F-12 culture medium, pre-cultivate for 24 hours under the condition of temperature 37 ℃, CO 25%, and then replace New culture fluid, and add parathyroid hormone (PTH, Parathyroid Hormone, final concentration 2×10-9M) and n-hexane extract (final concentration sees the table below, 440; 44 and 4.4 microgram / ml) in this culture fluid The culture was continued for 3 days under the same pre-culture conditions, and the 45Ca released into the culture solution was measured (measured value 1). This culture solution was renewed, and the culture was continued for another 3 days under the same conditions as the preculture, and 45Ca released into the culture solution was also measured (measurement value 2). The skull i...

Embodiment 3

[0032] The ethyl acetate extract of Achyranthes bidentata obtained in embodiment 1 is made into 5 kinds of concentrations described in the table below like embodiment 2, 440; 44; 4.4; 0.88 and 0.44 microgram / ml.

[0033]The extract was tested for its anti-bone resorption activity in the same manner as in Example 2, and the following results were obtained.

[0034] Ethyl acetate extract 45 Ca release rate (%) Normal group (without PTH and ethyl acetate extract) 50.81±4.36 PTH 100.00±5.56 PTH + ethyl acetate extract (440 μg / ml) 47.83±1.14 PTH + ethyl acetate extract (44 μg / ml) 48.10±1.56 PTH + ethyl acetate extract (4.4 μg / ml) 65.94±3.58 PTH + ethyl acetate extract (0.44 μg / ml) 79.87±2.31 PTH + ethyl acetate extract (0.88 μg / ml) 92.09±3.58

[0035] The results clearly show that the ethyl acetate extract of Achyranthes bidentata obtained in Example 1 has a strong inhibitory effect on hyperabsorption induced by parathyroid ...

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Abstract

The invention discloses a method of extracting triterpene from Achyranthes bidentata Bl. and use of the triterpene for anti-osteoporotics. The method comprises the following steps: dry roots of the Achyranthes bidentata Bl. are crushed; reflux extraction is performed on the crushed roots to obtain ethanol extract by ethanol heating; and the ethanol extract is extracted by esters such as ethyl acetate and the like for 1-4 times to obtain ester extracting solution, or the extract distilled water suspension extracted by the esters such as the ethyl acetate and the like is extracted by alcohol such as n-butyl alcohol and the like for 1-4 times to obtain alcohol extracting solution, finally the aqueous extract is obtained, and the obtained triterpene extract is used in anti-osteoporosis, wherein, the triterpene extract comprises new compounds such as found 28-deglucose Achyranthes saponin E dimethyl ester. The triterpene extract has good effect on anti- osteoporosis, and particularly an estrogen replacement therapy is unnecessary for female osteoporosis. The triterpene extract is a natural substitute drug with low side effect.

Description

[0001] The present invention is application number 03132105, application date 2003-06-27, a divisional application of triterpenoid extract named Achyranthes bidentata and its use in anti-osteoporosis drugs. 1. Technical field [0002] The present invention relates to the application of triterpenes (including triterpene glycosides) extract obtained from common traditional Chinese medicine Achyranthes bidentata (root of Achyranthes bidentata Bl.) as an anti-osteoporosis drug. 2. Background technology [0003] Osteoporosis is a common middle-aged and elderly disease characterized by decreased bone tissue mass and microstructural degradation, resulting in increased bone fragility and susceptibility to fractures. With the development of human society and the advancement of medical science and technology, the life expectancy of human beings has been greatly improved, making the aging of society increasingly serious. More and more people will suffer from osteoporosis. According to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61P19/10A61K31/56
Inventor 李建新门田重利
Owner NANJING UNIV
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