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Method for evaluating obesity controller

A control agent and obesity technology, applied in chemical instruments and methods, compound screening, animal/human protein, etc., can solve problems such as unclear changes in insulin secretion

Inactive Publication Date: 2009-09-30
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the changes in insulin secretion caused by carbohydrate and lipid intake and the relationship between these changes and obesity are still unclear

Method used

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  • Method for evaluating obesity controller
  • Method for evaluating obesity controller
  • Method for evaluating obesity controller

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Postprandial Insulin Secretion Increased by Ingestion of Carbohydrates and Lipids

[0063] Fasting mice (C57BL / 6J, male, 7-8 weeks old) were divided into groups, ten in each group, and the mice were orally given only 2 mg / g of body weight glucose ( Wako Pure Chemical Industries, Ltd.) as carbohydrates, or triolein (Sigma Corp.) of 0.5, 1.0 or 2.0 mg / g body weight was also administered together with carbohydrates as lipids, and the emulsion was made with egg yolk lecithin Obtained by emulsifying 0.02 mg / g body weight of lipids (carbohydrate+lipid 0, carbohydrate+lipid 1, carbohydrate+lipid 2, and carbohydrate+lipid 3, respectively). The composition of the administered substances is shown in Table 1. Before the administration and 10 minutes and 30 minutes after the administration, blood samples were taken from the orbital vein of each mouse, the blood insulin level was measured, and the area under the curve (AUC) of the graph was calculated. Measurement of in...

Embodiment 2

[0071] Example 2: Correlation between postprandial insulin secretion and obesity

[0072] (1) Determination of postprandial insulin secretion

[0073] Glyceryl triolein (Sigma Corp.) was used as the lipid and starches (gelatinized, obtained from sweet potato, cassava, potato, kudzu, sago, waxy corn, from Nippon Starch Chemical Co., Ltd.) as the carbohydrate.

[0074] Fasting mice (C57BL / 6J, male, 10 to 11 weeks old) were divided into groups of 4 to 5, and the mice were orally administered carbohydrates at 2 mg / g body weight using a probe, or together with carbohydrates Triolein plus 0.08 mg / g body weight was also administered at 2 mg / g body weight. After 10 minutes of administration, blood was collected from the orbital vein of each mouse, and blood glucose level and blood insulin level were measured. Blood glucose levels were measured using a small blood glucose meter (glucose dehydrogenase / potential difference measurement, Roche Diagnostics, Inc.). Insulin levels were me...

Embodiment 3

[0086] Example 3: Inducibility of hypoinsulin secretion by glycogen

[0087] Triolein (Sigma Corp.) was used as lipid, while glucose (WakoPure Chemical Industries, Ltd.) or glycogen (from sweet corn, QP Corp.) was used as carbohydrate.

[0088] Fasting mice (C57BL / 6J, male, 10 to 11 weeks old) were divided into groups, 9 to 10 in each group, and 2 mg / g body weight of glucose was orally administered to the mice using a probe; Together with 2 mg / g body weight of triolein obtained by emulsifying 0.02 mg / g body weight of lipid with egg yolk lecithin (glucose, and glucose+lipid, respectively); 2 mg / g body weight glycogen; or triolein (TAG) at 2 mg / g body weight was also given together with glycogen in the form of an emulsion obtained by emulsifying 0.02 mg / g body weight lipid with egg yolk lecithin (respectively for glycogen, and glycogen + lipid). The compositions of the administered substances are shown in Table 4. Before the administration, and 10 minutes and 30 minutes after...

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Abstract

The present invention discloses a method for efficiently evaluating or selecting an obesity controlling substance, a blood insulin regulating substance or a blood sugar regulating substance, is provided. A method for evaluating or screening an obesity controller, a blood insulin regulator or a blood sugar regulator, the method including administering a carbohydrate and a lipid to an animal, and evaluating or selecting a substance which decreases or increases insulin secretion, is also provided.

Description

technical field [0001] The present invention relates to a method for evaluating or screening obesity control agents, blood insulin regulators, or blood sugar regulators. Background technique [0002] Obesity is a condition in which there is an abnormal increase in fatty tissue and is believed to lead to lifestyle-related diseases such as diabetes, heart disease and arteriosclerosis. In modern society, obesity has become a serious problem due to high-fat diet, excessive exercise or lack of exercise, and finding substances that have a preventive / improving effect on obesity has also become an important issue. [0003] As a method for selecting an obesity-improving agent, the following methods have been reported: a method of measuring the amount of adiponectin (adiponectin) expressed by mature adipocytes (Patent Document 1); measuring the activity of UCP-2, which is one of the uncoupling proteins, or transcription / mRNA method (Patent Document 2); method of measuring the express...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74C07K14/62
CPCG01N2800/042G01N33/74C07K14/62G01N2800/044G01N2500/00G01N33/5085G01N2333/62
Inventor 下丰留玲铃木淳子竹野菜奈美福冈大介水野智仁目黑真一
Owner KAO CORP
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