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Extracting method of beef liver metallothionein

A metallothionein and extraction method technology, applied in the field of metallothionein preparation, can solve the problems of inability to meet the needs of national economic development, limited donors, and high cost, and achieve wide sources of inducers, large feeding amount, and high cost. low effect

Inactive Publication Date: 2009-11-18
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the existing products on the market are metallothionein containing zinc extracted from bacteria, snails and rabbit livers. Due to limited donors and high cost, they cannot meet the needs of national economic development.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] ①Induced synthesis

[0029] Select healthy newborn dairy cow calves, use the prepared ZnSO 4 ·7H 2 O solution was induced by intramuscular injection in the neck, once every 1 d, for a total of 5 inductions. Each dose is 5, 10, 20, 50, 100mg / kg, accumulatively 185mg / kg.

[0030] Using cattle as donors, the amount of metallothionein produced in the liver after 5 inductions was 8.60 mg / g, which was significantly higher than the usual amount of metallothionein (2.62 mg / g) produced by 3 inductions and the amount of metallothionein produced by 4 inductions. The amount of metallothionein produced by the first induction (4.77mg / g); and the amount of metallothionein produced by the 7th induction (9.49mg / g)) and the amount of metallothionein produced by the 9th induction (9.61mg / g) The difference is not significant and saves induction time and cost.

[0031] ② Quantitative detection

[0032] According to the conditions, choose one of the following methods:

[0033] A. Sulf...

Embodiment 2

[0044] The basic steps are the same as in Example 1, except that the total induction is 7 times, and each dose is 5, 10, 20, 50, 100, 150, 200 mg / kg, accumulatively 535 mg / kg; and in the separation and purification step, at -80 Freeze at ℃ for 25-30 hours, and the obtained lyophilized sample is pure metallothionein with a content of more than 96%. 8mg-10mg of metallothionein can be extracted from every 1g of bovine liver.

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Abstract

The invention relates to an extracting method of beef liver metallothionein, which comprises the following steps: providing a healthy calf and carrying out 5-7 times of inducements with progressive dosages by neck muscle injection ZnSO4 solution to synthesize the metallothionein; detecting quantitively and requiring the content of the metallothionein at every 1g beef liver to be more than or equal to 7mg; mashing the beef liver which is eligible by detection and carrying out pretreatment and coarse extraction; and finally carrying out separation and purification to obtain freeze-dried powders, namely, pure metallothionein with the content more than 96 percent; thus, the metallothionein synthesized by the beef liver induced by zinc has the advantages of low cost, good effect, high output, wide inducer source, no side effects, and the like and develops a new way for enlarging the source of the metallothionein as an important bioactive substance and accelerating the research and the application of the metallothionein.

Description

Technical field: [0001] The invention relates to a method for preparing metallothionein, in particular to a method for extracting bovine liver metallothionein. Background technique: [0002] Metallothionein is an important biologically active substance with a wide range of physiological and biological functions, especially in scavenging free radicals in the body, detoxifying heavy metals, anti-oxidation and anti-stress, so it is used as an additive and Drugs for the treatment of certain diseases have been widely used in various fields such as medicine, food processing, and nutrition, and are expected to play an important role in promoting animal health, increasing the yield, nutritional value, and quality and safety of animal products. [0003] Zinc-containing metallothionein (Zn-MT) is a regular tetrahedral metal thiol chelating group with a relatively stable crystal structure, but it can still activate many enzymes with Zn as a prosthetic group by providing Zn. Therefore,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
Inventor 张彬李丽立王莹
Owner HUNAN AGRICULTURAL UNIV
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