Method for artificially hastening parturition of American reeves shads, inseminating and hatching germ cells
A technology of artificial induced spawning and American shad, applied in climate change adaptation, fish farming, application, etc., can solve the problems of low fish fry yield and poor quality, and achieve the goal of solving large-scale production problems, reducing damage, and improving fertilization rate Effect
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Embodiment 1
[0027] Embodiment 1: a kind of American shad artificial oxytocin, insemination and hatching method of fertilized eggs of the present invention adopt the following hatching steps:
[0028] Location: Base of Yangtze River Rare Fish Conservation Research Center
[0029] 1. Artificial induced labor process
[0030] ①Preparation process Catch and select American shad with good maturity in 8-acre breeding ponds, and transport them to an area of 20m2 through deep well water with a salinity of 5‰ 2 , temporarily raised in a cement pool with a water depth of 0.8m; and equipped with an oxytocin needle and a thin towel of 0.4×0.2m.
[0031] ② Artificial induction of labor using luteinizing hormone-releasing hormone analogues (LRH-A 2 ) and fish oxytocin auxiliaries (Ningbo Hormone Products Co., Ltd.) are compatible to oxytocin broodstock. Two injections at the base of the dorsal fin: the first time, the female shad injection dose: 60μg LRH-A 2 +10mg fish oxytocin adjuvant / Kg, no in...
Embodiment 2
[0040] Embodiment 2: a kind of American shad artificial induced labor, insemination and hatching method of fertilized eggs of the present invention adopt the following hatching steps:
[0041] Location: Jiangyin Shengang Sanxian Breeding Co., Ltd.
[0042] 1. Artificial induced labor process
[0043] ① During the preparation process, American shad with good maturity was caught and selected in 8-acre breeding ponds, and transported to an area of 20m2 by deep well water with a salinity of 5‰ 2 , temporarily raised in a cement pool with a water depth of 0.9m; and equipped with an oxytocin needle and a thin towel of 0.4×0.2m.
[0044] ② Artificial induced labor using luteinizing hormone-releasing hormone analogs (LRH-A 2 ) and fish oxytocin auxiliaries (Ningbo Hormone Products Co., Ltd.) are compatible to oxytocin broodstock. Two injections at the base of the dorsal fin: the first time, the female shad injection dose: 60μg LRH-A 2 +10mg fish oxytocin adjuvant / Kg, no injectio...
Embodiment 3
[0054] Embodiment 3: a kind of American shad artificial induced labor, insemination and hatching method of fertilized eggs of the present invention adopt the following hatching steps:
[0055] Location: Jiangyin Fisheries Guidance Station Base
[0056] 1. Artificial induced labor process
[0057] ①Preparation process Catch and select American shad with good maturity in 8-acre breeding ponds, and transport them to an area of 20m2 through deep well water with a salinity of 5‰ 2 , temporarily raised in a cement pool with a water depth of 1.0m; and equipped with an oxytocin needle and a thin towel of 0.4×0.2m.
[0058] ② Artificial induction of labor using luteinizing hormone-releasing hormone analogues (LRH-A 2 ) and fish oxytocin auxiliaries (Ningbo Hormone Products Co., Ltd.) are compatible to oxytocin broodstock. Two injections at the base of the dorsal fin: the first time, the female shad injection dose: 60μg LRH-A 2 +10mg fish oxytocin aid / Kg, no injection for male fis...
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