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Preparation method and application of galloyl glucose compounds

A technology of galloyl and glucose, which is applied in the field of preparation and application of galloyl-glucose compounds, can solve the problems that the anti-hypoxia active ingredients have not been elucidated, and the anti-hypoxia and anti-tumor activities of galloyl-glucose compounds have not been reported. , to reduce the symptoms of discomfort

Inactive Publication Date: 2010-03-31
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the hypoxic protective effect of total flavonoids of South Suanzizao fruit has also been documented (Li Zengxi et al.; Protective effect of total flavonoids of Guangzao jujube on animal hypoxia tolerance and acute myocardial ischemia: Chinese Herbal Medicine, 1984, Volume 15, No. 6, pages 25-27), but the anti-hypoxic active ingredient has not been elucidated
The anti-hypoxic and anti-tumor activities and uses of the galloyl glucose compounds isolated and prepared from the extract of Suanzizao jujuba and their uses have not been reported so far

Method used

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  • Preparation method and application of galloyl glucose compounds
  • Preparation method and application of galloyl glucose compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1. Separation and preparation of compounds I~V

[0023] Extraction and Extraction Separation of Raw Material Medicinal Plant Jujube

[0024] 3.2kg of dry bark of Choerospondias axillaries (Roxb.) Burtt.et Hill. (collected in August 1999 from Mengla, Yunnan) was pulverized and extracted with commercially available medical 95% (v / v) ethanol at room temperature, each time Soak in 25 liters of ethanol for 7 days, extract 4 times in total, combine the extracts, concentrate and dry under reduced pressure to obtain 750 g of ethanol extract. 750 g of the ethanol extract was suspended in 3 liters of water, extracted successively with chloroform, ethyl acetate, and n-butanol, each solvent was used for 3 liters each, extracted 4 times respectively, the same extracts were combined, concentrated and dried under reduced pressure, and obtained 60 g of the chloroform extract, 310 g of the ethyl acetate extract, 300 g of the n-butanol extract, and 80 g of the aqueous layer r...

Embodiment 2

[0039] Embodiment 2. Anti-hypoxic activity test

[0040] Cell line and cell culture: The rat pheochromocytoma PC12 cell line was used for the activity test, and the cells were filled with DMEM medium containing 10% newborn calf serum and 100 μg / mL of penicillin and streptomycin at 37°C. CO 2 Routine culture and subculture maintenance in an incubator with 95% air.

[0041] Test sample and sample solution preparation: Compounds I, IV and V in Example 1. Accurately weigh an appropriate amount of the sample to be tested, dissolve it in DMEM medium containing 10% newborn calf serum and 100 μg / mL of penicillin and streptomycin, and prepare sample solutions of 50 μg / mL, 100 μg / mL and 200 μg / mL for For activity testing.

[0042] Activity test method: take PC12 cells in the logarithmic growth phase, and use fresh DMEM medium containing 10% newborn calf serum and 100 μg / mL of penicillin and streptomycin to prepare a cell density of 1 × 10 per ml. 5 The cell suspension was inoculated...

Embodiment 3

[0048] Example 3. Antitumor Activity Test

[0049] Cell line and cell culture: The human leukemia K562 cell line was used for the activity test, and the cells were filled with RPMI-1640 medium containing 10% fetal bovine serum and 100 μg / mL of penicillin and streptomycin at 37°C with 5% CO 2 Routine culture and subculture maintenance in an incubator with 95% air.

[0050] Test sample and sample solution preparation: Compounds I-V in Example 1. Precisely weigh an appropriate amount of the sample to be tested, dissolve it in methanol, and prepare a 10 mg / mL sample solution for the activity test.

[0051] Activity test method: collect K562 cells in the logarithmic growth phase, and use fresh RPMI-1640 medium to prepare a cell density of 2×10 5 cells / mL of cell suspension, seeded in 96-well plate, 200 μL per well. The inoculated cells were incubated at 37°C in 5% CO 2 After culturing in a cell incubator with 95% air for 4 hours, add 2 μL of sample solutions of different concen...

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Abstract

The invention relates to a method for extracting and purifying galloyl glucose compounds contained in medicinal plant choerospondias axillaries, and an application of the galloyl glucose compounds asactive components in preparing antitumor drugs or antihypoxic drugs or health products and other functional foods.

Description

Technical field: [0001] The invention relates to a preparation method of galloyl glucose compounds contained in the extract of the medicinal plant Jujube jujuba, and the use of these compounds for preparing functional foods such as anti-tumor or anti-hypoxia drugs and anti-hypoxia health care products. Background technique: [0002] Oxygen is indispensable in human life activities, and oxygen molecules play an important role in the energy metabolism of tissues and cells in the body. A variety of factors can lead to insufficient oxygen supply to human tissues and cells, such as certain diseases or being in a special hypoxic environment (such as plateau), etc., and hypoxia can in turn cause cell and tissue damage and even various serious diseases, such as Severe respiratory diseases, concurrent cardiovascular and cerebrovascular diseases, and low immune function. Severe hypoxia is often the direct cause of death in many related critically ill patients. Therefore, the researc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7024A61K36/22A61P35/00A61K131/00
Inventor 崔承彬李长伟蔡兵韩冰李明明范明
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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