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Ephedra total polysaccharide extractive and preparation method and medical application thereof

A technology of total polysaccharides and extracts, applied in the field of ephedra total polysaccharides extract and its preparation, can solve problems such as unaffordable for patients, limitations of treatment objects and programs, lack of selectivity and specificity, etc. The effect of inhibiting activity

Active Publication Date: 2010-05-12
匡海学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] From the current point of view, the clinically used immunosuppressants are mainly chemically synthesized drugs, which still have the disadvantages of extensive and strong toxic and side effects, limited treatment objects and programs, and most of them lack selectivity and specificity. Both abnormal and abnormal immune responses have inhibitory effects, and the inhibitory effect on the primary immune response is stronger, and the inhibitory effect on the secondary immune response is weaker
The long-term application of these chemically synthesized drugs can easily reduce the body's resistance and induce infection, tumor, liver and kidney toxicity, bone marrow suppression, metabolic disorders, and nervous system dysfunction.
In addition, the high price often makes it difficult for patients to afford

Method used

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  • Ephedra total polysaccharide extractive and preparation method and medical application thereof
  • Ephedra total polysaccharide extractive and preparation method and medical application thereof
  • Ephedra total polysaccharide extractive and preparation method and medical application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Take about 100 kg of ephedra section, add 5 times the volume of distilled water, decoct and extract 3 times, each time for 3 hours, filter, combine the filtrate, concentrate to 0.5 times the volume of the medicinal material, adjust the alcohol content to 80% with 95% ethanol, and statically Place overnight, centrifuge at 2000 rpm per minute, and wash the precipitate with 3 times the amount of absolute ethanol and acetone in turn to obtain the precipitate. Take the precipitate and add 0.5 times the weight of distilled water to redissolve, and load the molecular weight range to 3.0×10 3 -1.2×10 4 Dialyze in the dialysis bag for 48 hours with running water and 48 hours with distilled water, freeze-dry the dialysate to obtain crude polysaccharides; remove the pigment and protein in the total polysaccharides through weak acid and weak base type anion and cation exchange resins (Amberlite FPA90Cl+Amberlite IRC84), and obtain refined ephedra The polysaccharide is 3960g, and t...

Embodiment 2

[0051] About 500g of the middle ephedra segment, add 10 times the volume of distilled water, decoct and extract 4 times, 2 hours each time, filter, combine the filtrate, concentrate to 1 times the volume of the medicinal material, adjust the alcohol content to 85% with absolute ethanol, and let stand Overnight, centrifuge at 2000 rpm per minute, and wash the precipitate with 4 times the amount of absolute ethanol and acetone in sequence to obtain the precipitate. Take the precipitate and add 2 times the weight of distilled water to redissolve, and load the molecular weight range to 3.0×10 3 -1.2×10 4 Dialyze in the dialysis bag for 10 hours with running water and 10 hours with distilled water; freeze-dry the dialysate to obtain crude polysaccharide; pass through weak acid and weak base anion and cation exchange resins (Amberlite FPA90C1+Amberlite IRC84) to remove the pigment and protein in the total polysaccharide to obtain refined ephedra The polysaccharide is 22.7g, and the...

Embodiment 3

[0053] About 500g of the horsetail ephedra segment, add 10 times the volume of 35% ethanol to reflux and extract twice, each time for 2 hours, filter, combine the filtrate, recover the solvent under reduced pressure to 0.2 times the volume of the medicinal material, and adjust the alcohol content to 90%, let it stand overnight, centrifuge at 2000rpm, and wash the precipitate with 2 times the amount of absolute ethanol and acetone in turn to obtain the precipitate. Take the precipitate and add 1 times the weight of distilled water to redissolve, and load the molecular weight range of 3.0×10 3 -1.2×10 4 Dialyze in the dialysis bag for 28 hours with running water and 28 hours with distilled water; freeze-dry the dialysate to obtain crude polysaccharide; pass through weak acid and weak base type anion and cation exchange resins (Amberlite FPA90Cl+Amberlite IRC84) to remove the pigment and protein in the total polysaccharide to obtain refined ephedra The polysaccharide is 20.5g, an...

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Abstract

The invention discloses an ephedra total polysaccharide extractive and a preparation method and a medical application thereof. The ephedra total polysaccharide in the invention is acid total polysaccharide, calculated by glucose, and the weight percent content of the acid total polysaccharide accounts for 45% or over 45% of the total weight of ephedra total polysaccharide extractive. In the invention, a large number of pharmacological experiments discover that the ephedra acid total polysaccharide extractive has prominent immunosuppressive activity, has no toxic and side effects, can be prepared to drugs used as immunosuppressant or can be used as bulk drug for preparing other immunosuppressive drugs, and can be used for curing various diseases related to over-immunity, comprising allergic asthma, chronic nephritis, rheumatoid arthritis, systemic lupus erythematosus, body rejection caused by organ transplantation and other diseases related to over-immunity.

Description

technical field [0001] The present invention relates to an extract of effective parts of traditional Chinese medicine, in particular to an extract of total polysaccharides from ephedra ephedra and a preparation method thereof. The present invention also relates to the extract of total polysaccharides from ephedra ephedra as an immunosuppressant or for treating various diseases related to excessive immunity The medicinal use belongs to the field of extracts from effective parts of traditional Chinese medicines. Background technique [0002] The body's immune system is stable and dysfunctional, and the overexpression of immune function will produce immune rejection to its own tissues, causing physiological dysfunction, leading to various tissue damage and pathological damage, and clinical symptoms, which are called autoimmune diseases, such as rheumatoid Arthritis (global incidence is about 0.1-1%, my country is about 0.32-0.36%), systemic lupus erythematosus (global incidence ...

Claims

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Application Information

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IPC IPC(8): A61K36/17A61P37/06A61P11/06A61P13/12A61P29/00A61P19/04A61P37/02C08B37/00A61K135/00
Inventor 匡海学王秋红夏永刚王艳宏杨炳友
Owner 匡海学
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