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Technology for efficiently extracting radix puerariae starch and radix puerariae total isoflavone

A technology of pueraria starch and isoflavones, which is applied in the direction of fermentation, can solve the problems of affecting product quality, phenolic substances are prone to browning, difficulty, etc., and achieves the effects of improving the utilization rate of raw materials, avoiding the phenomenon of browning, and promoting rapid degradation.

Inactive Publication Date: 2012-05-30
重庆正里元实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

China's Ministry of Health announced in 1988 that it is both edible and medicinal plants. In recent years, the planting area has developed rapidly, but the processing and utilization of Pueraria lobata is still in its infancy. Extraction, or more important than the extraction of Pueraria total isoflavones, lacks the technology that can simultaneously extract Pueraria powder and Pueraria total isoflavones, such as the Chinese patent of "Pueraria powder (Gudzu powder) and its manufacturing process" whose application number is 93104516.9, The purpose of this process is to extract pueraria powder, and the extraction of flavonoids in pueraria is abandoned when extracting dry starch from fresh pueraria; the Chinese patent of "A New Method for Extracting High-purity Medicinal Puerarin" with application number 99115791 , the shortcoming of this new extraction method is: only the flavonoids in Puerariae root are single-extracted, promptly the extraction of Puerariae powder is abandoned when extracting the active ingredient of flavonoids from Puerariae slices, meanwhile, this method has adopted ethyl acetate and n-butyl Alcohol, methanol and other organic solvents with certain neurotoxicity, in the subsequent processing of the product, the removal of the organic solvent also increases the difficulty and complexity of the process, which brings difficulty to the increase of the added value of the product
However, the crude fiber content of the fresh pueraria root skin is high, and it contains pectin and protein. If the pueraria root is not thoroughly crushed and ground, the free rate of its starch will not be ideal. These are the efficient extraction of pueraria starch and total isoflavones of pueraria. At the same time, the activity of polyphenol oxidase in fresh kudzu root is higher, so that the phenolic substances in kudzu root are prone to browning, which affects the quality of products
[0004] The invention patent of "Separation and Comprehensive Extraction of Pueraria Root Powder and Flavonoids Using Metal Nano-membrane" with the application number of 021279661 provides a method for comprehensively extracting Pueraria root powder and total isoflavones from Pueraria root powder. Using ultrafiltration membrane technology, the separation efficiency of kudzu root powder is low, the yield is low, and it is not suitable for large-scale batch production, and this method focuses on the extraction of total isoflavones of kudzu root, ignoring the retention of the total isoflavones content of kudzu root powder. , the effective content of kudzu root powder produced jointly is not high, only similar to ordinary sweet potato starch, with low nutritional added value
[0005] The invention patent of "A Method for Extracting Starch with High Total Flavonoid Content from Pueraria Root" with application number 200410026498.5 discloses a method for extracting starch with high total flavonoid content from Pueraria Root. The color and the color are soaked in alkaline substances and then separated. The process of the invention is complicated, the yield of Pueraria starch and the extraction efficiency of flavonoids are not high, which is not conducive to industrial production, and the use of color-protecting agents not only pollutes the environment to a certain extent, but also It is also inevitable that the product will contain additives

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The process for efficiently extracting Pueraria starch and Pueraria total isoflavones of the present embodiment comprises the following steps:

[0029] a. After mixing 10 kg of kudzu root with 20 kg of water, carry out initial crushing and filing to obtain the mixed material, then apply a high pressure of 130 MPa to the mixed material, and the pressure application time is 10 minutes. After testing, after applying the high pressure, The activity of polyphenol oxidase in the mixed material was reduced to 34%;

[0030] b. adjust the temperature of the mixed material obtained in step a to 33-35° C. and add cellulase, and after 1 hour, file the mixed material again to obtain a slurry mixture, and the weight of cellulase is 1% of the weight of the mixed material;

[0031] c. adjust the pH value of the slurry mixture obtained in step b to be 8, adjust the pH value to 5 after 1 hour, and separate the slurry mixture after 0.5 hour to obtain slurry and kudzu root residue;

[003...

Embodiment 2

[0041] The process of efficiently extracting Pueraria starch and Pueraria total isoflavones of the present embodiment: comprises the following steps:

[0042] a. After mixing 10 kg of kudzu root with 30 kg of water, carry out initial crushing and filing to obtain the mixed material, then apply high pressure to the mixed material, the size of the high pressure is 300MPa, and the number of times of application is 2 times, each 15mins, After an interval of 3 minutes, after applying high pressure, the activity of polyphenol oxidase in the mixed material was detected to be reduced to 28% of the original;

[0043] b. adjust the temperature of the mixed material obtained in step a to 35-38° C. and add cellulase. After 1.5 hours, the mixed material is again ground to obtain a slurry mixture. The weight of the cellulase is the weight of the mixed material 3%;

[0044] c. adjust the pH value of the slurry mixture obtained in step b to be 9, adjust the pH value to 6 after 1.5 hours, and...

Embodiment 3

[0054] The difference between the technology of the present embodiment and embodiment 2 is:

[0055] In step a, the high pressure is 260MPa, and the number of times of application is 3 times, 20 minutes each time, with an interval of 3 minutes in between. After the high pressure is applied, the activity of polyphenol oxidase in the mixed material is reduced to 15% of its original value;

[0056] In step b, the temperature of the mixed material is adjusted to 33-36°C, and the weight of cellulase is 2% of the weight of the mixed material;

[0057] In step c, the pH adjustment method is: first adjust the pH value of the slurry mixture to 8, and then adjust the pH value to 5 after 1.5 hours;

[0058] In step d, the weight of water added to the slurry obtained in step c is 2 times of its own weight;

[0059] In step i, pueraria slag is immersed in 3 times the weight of ethanol solution and subjected to intermittent microwave treatment at 65° C., the mass percentage of ethanol in t...

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Abstract

The invention discloses a technology for efficiently extracting radix puerariae starch and radix puerariae total isoflavone, comprising the following steps: breaking, draw filing, pH value adjustment and dipping, slag slurry separation, radix puerariae starch extraction, concentration, drying and radix puerariae total isoflavone recovery in process water; applying high pressure to the mixture obtained by draw filing to passivate polyphenol oxidase; adding cellulose enzyme to degrade the cellulose to ensure starch ionization rate; further setting the dipping time after the pH value of the slagslurry mixture is adjusted so as to not only ensure the full extraction of total isoflavone but also avoid character change of the radix puerariae starch due to improper dipping time; and further recovering the radix puerariae total isoflavone by the air flotation of dissolved air in the whole process so as to ensure the extraction rate of the radix puerariae total isoflavone. The whole process flow is simple, has easily controlled condition, protects environment, efficiently extracts radix puerariae starch and radix puerariae total isoflavone and greatly improves economic benefit.

Description

technical field [0001] The invention relates to a food processing technology, in particular to a technology for efficiently extracting kudzu root starch and total isoflavones from kudzu root. Background technique [0002] Pueraria lobata is the tuberous root of Pueraria genus Fabaceae, which was first recorded in "Shen Nong's Herbal Classic" in the Han Dynasty of my country. Pueraria radix is ​​rich in starch and flavonoids (namely daidzin, puerarin, puerarin), and has high edible value and medical and health value. my country's Ministry of Health announced in 1988 that it is both edible and medicinal plants. In recent years, the planting area has developed rapidly, but the processing and utilization of Pueraria lobata is still in its infancy. Extraction, or more important than the extraction of Pueraria total isoflavones, lacks the technology that can simultaneously extract Pueraria powder and Pueraria total isoflavones, such as the Chinese patent of "Pueraria powder (Gudzu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12P17/06
Inventor 李正元
Owner 重庆正里元实业有限公司
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