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Method for identifying quality of bird's nest

A technology of free sialic acid and bird's nest, which is used in measurement devices, material separation, instruments, etc. to achieve high sensitivity

Active Publication Date: 2012-09-26
SHENZHEN MANNAY COSMETIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention of the bird’s nest plays a buffer role in solving the current contradiction between supply and demand of bird’s nest. As the market’s demand for natural bird’s nest continues unabated, the price of similar products has increased by dozens of times.
At present, the international and domestic regulatory authorities do not have an objective and scientific testing standard for the relationship between the value of different types of bird’s nests in the market and their own quality. Especially in the domestic market, the prices of bird’s nests are randomly marked and the quality is inconsistent, causing certain confusion.

Method used

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  • Method for identifying quality of bird's nest

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The determination of free sialic acid content in the dried bird's nest of embodiment 1

[0047]A method for identifying the quality of bird's nest, using high performance liquid chromatography triple quadrupole quality (HPLC-QQQ) to measure the free sialic acid content in the bird's nest, comprising the following steps:

[0048] (1) Sample processing

[0049] Accurately weigh 10mg of dried bird's nest sample, add 1ml of pure water, then ultrasonic for 10 minutes, leave for 5 minutes (at 14,000 revolutions per minute), take 500μl of the supernatant into the sample bottle, and get ready;

[0050] (2) Liquid chromatography separation

[0051] The conditions are as follows:

[0052] ①Instrument: HPLC column: Eclipse XDB-C 18 (2.1×100mm, 3.5μm) chromatographic column

[0053] ② Chromatography conditions: flow rate 0.4mL / min, injection volume 2μL.

[0054]

[0055] (3) Mass spectrometry detection

[0056] The conditions are as follows:

[0057] Atomization pressure:...

Embodiment 2

[0077] Embodiment 2 Methodological verification of the inventive method

[0078] a. Precision test

[0079] Accurately measure 0.10mL of 0.1mM N-acetyl-D-neuraminic acid (N-Acetyl-D-neuraminic acid) standard solution, and measure it according to the method under the item "Liquid Chromatography Separation and Mass Spectrometry Detection" in Example 1, continuous It was measured 5 times, and its RSD was 4.24%.

[0080] b. Repeatability test

[0081] Get 5 parts of bird's nests of the same batch simultaneously, prepare standard solution under the item "preparation of standard solution" of embodiment 1, then carry out by the method under the item of embodiment 1 "sample processing" and "liquid chromatography separation and mass spectrometry detection" Measure and calculate the content of free sialic acid, the average value is 782.1ng / kg, RSD is 3.71%. It is proved that the method of the present invention has good repeatability.

[0082] Table 1 Results of repeatability test of...

Embodiment 3

[0087] Example 3 The present invention identifies the sample processing condition screening of bird's nest quality method

[0088] (1) Screening of extraction solvent

[0089] Take 10 mg of dried bird's nest sample, add 1 ml of purified water and 1 ml of methanol respectively, then ultrasonic for 10 minutes, centrifuge for 5 minutes (at 14,000 revolutions per minute), take 500 microliters of the supernatant and put it into a sample bottle, according to the example The method under 1 " liquid chromatography separation and mass spectrometry detection " item is measured, and the result is as follows figure 2 (n=3). It shows that using water as the extraction solvent is better than methanol, which is more in line with reality.

[0090] (2) Screening of dissociation methods

[0091] Accurately weigh four samples of 10 mg of dried bird's nest, add 1 ml of pure water to each, soak for 5 minutes, 10 minutes, 20 minutes and ultrasonically extract for 10 minutes, centrifuge for 5 mi...

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Abstract

The invention discloses a method for identifying the quality of a bird's nest, which identifies the quality of the bird's nest by using a high performance liquid chromatography triple tandem quadrupole mass spectrometry (HPLC-QQQ) to determine the dissociative sialic acid content of the bird's nest, and comprises the steps of sample treatment, high performance liquid chromatography separation andmass spectrometric detection. The method has the advantages of high speed, accuracy and high sensitivity, thereby being a good method for identifying whether the bird's nest is true or not and whether the quality is good or not. Compared with the traditional identifying method based on appearance and properties, the method of the invention is more objective and more scientific.

Description

technical field [0001] The invention relates to a method for identifying the quality of bird's nest, in particular to a method for measuring the content of free sialic acid in bird's nest to identify the quality of dried bird's nest and instant bird's nest. Background technique [0002] Bird's nest is a variety of birds of the swift family (Apodidae) swiftlet genus (Collocalia). The secreted saliva or saliva mixed with its down feathers condenses on the cliffs and cliffs to form nests. The main production areas are Indonesia, Malaysia, Vietnam, etc. According to the "Compendium of Materia Medica": "Bird's nest is sweet and flat, nourishes lung yin, reduces phlegm and relieves cough, nourishes and clears, and is a holy medicine for recuperating fatigue. All diseases are caused by lung deficiency. Those who can't clear up the downward flow can be cured with this." Chinese medicine believes that bird's nest: "nourishing yin and moistening dryness, nourishing qi and invigoratin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/72
Inventor 詹华强董婷霞陈嘉伦王铁杰
Owner SHENZHEN MANNAY COSMETIC
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