Method and device for separating sperms

Abstract

The invention discloses simple and high-efficient method and device for separating sperms. The device consists of an Eppendorff centrifugal tube, a separating tube and a rubber ring. The method comprises the steps of: reasonably controlling the level height of culture solution; and culturing in a 5 percent CO2 culture container at 37 DEG C for 15minutes for separating the sperms. The concentration of the sperms can be adjusted by adopting a low-speed centrifugal method; about 95 percent of obtained sperms have athletic ability; and the collected solution generally has no blood cells. The invention is suitable for an in vitro capacitation experiment.

Description

technical field [0001] The invention relates to a method and device for separating sperm. Background technique [0002] Fertilization is the process of combining sperm and egg, which is an important phenomenon of life. Mammalian sperm cannot combine with eggs immediately after ejaculation, and must stay in the reproductive tract of female animals for a period of time, or be cultured in appropriate culture medium for a certain period of time before they have the ability to combine with eggs. This process is called capacitation. Capacitation is a very critical part of natural reproduction. At present, the mechanism of capacitation has not been elucidated, and most of the data are obtained by culturing sperm in appropriate culture medium in vitro. Mice and rats are the most used animals in the study of capacitation. Generally, the sperm from the tail of the epididymis of mice or rats is used to study the capacitation mechanism. There are two problems now. 1. When collecting...

AI Technical Summary

Problems solved by technology

1. When collecting sperm from the tail of the epididymis, it is difficult to completely remove the small blood vessels on the surface of the epididymis, and there are always some blood cells in the obtained sperm solution

2. A small amount of dead or poorly motile sperm in the sperm solution

[0003] The disclosed inventive device for sperm separation also includes "a double-tube type floating screening sperm tube" (Patent No. 200920019260.8), which includes two vertical tubes, the first vertical tube communicates with the second vertical tube through a horizontal tube, and the first vertical tube An annular bump is set on the lower side wall of the ring bump, and the included angle between the bump side wall of the ring bump and the horizontal plane is α, 60 degrees ≤ α ≤ 85 degrees, and the diameters of the first riser and the second riser are equal, both are D , the diameter of the horizontal tube is d, 2d=D, the making of this device is more complicated than the device of the present invention, and it is not practical

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