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Method for evaluating PBDEs xenoestrogen effect by using gobiocyprisrarus gene expression

A rare carp, gene expression technology, applied in the field of water environment evaluation, can solve the problems of longer time required for sperm and egg maturation, fewer sperm cells, etc.

Inactive Publication Date: 2010-11-03
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Estrogen-like effects refer to some physiological changes or structural changes caused by acting on target organs similar to estrogen, especially some feminization phenomena, such as fewer sperm cells, longer time required for sperm egg maturation, etc. At present, the current research generally evaluates the degree of estrogen-like effects of environmental toxicants on organisms through vitellin (Li et al., 2005), and there is no report using fish to evaluate the estrogen-like effects of PBDEs
So far, no public reports have been found using the rare gobi and CYP19 gene as a method to evaluate the estrogen-like effects of brominated flame retardants (PBDEs)

Method used

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  • Method for evaluating PBDEs xenoestrogen effect by using gobiocyprisrarus gene expression
  • Method for evaluating PBDEs xenoestrogen effect by using gobiocyprisrarus gene expression
  • Method for evaluating PBDEs xenoestrogen effect by using gobiocyprisrarus gene expression

Examples

Experimental program
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Effect test

Embodiment Construction

[0028] 1. Set up four fish ponds with the same environment, put polybrominated diphenyl ethers (polybrominated diphenyl ethers, PBDEs for short) water with a concentration of 1ppb in the first fish pond, which is called the first sample pond, and in the second fish pond Put water with PBDEs concentration of 10ppb in the pond, called the second sample pond, put water with PBDEs concentration of 100ppb in the third aquarium pond, called the third sample pond, and put it in the fourth aquarium pond The clear water without adding any medicine is called the sample pool of the control group;

[0029] 2. Put the rare squid fry that hatched for 2-3 days into four fish ponds respectively, and raise them under the same breeding conditions;

[0030] 3. After feeding for 3-5 months, fish out the same number of rare minnows from the four fish ponds, generally 30-200;

[0031] 4. All the rare goby fished in the first sample pool are called the first sample type, all the rare goby fished in...

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Abstract

The invention discloses a method for evaluating a PBDEs xenoestrogen effect by using gobiocyprisrarus gene expression, and relates to the evaluation of the influence of water environmental pollution on hydrobios. In the method, the expression quantity of CYP19b genes in the brain tissue and gonad tissue of gobiocyprisrarus cultivated in water with different amounts of PBDEs is detected to illustrate that the PBDEs content of the water is higher, the xenoestrogen effect is more obvious, so that the method for evaluating the PBDEs xenoestrogen effect is obtained.

Description

Technical field: [0001] The invention relates to the evaluation of water body environment, in particular to the evaluation of the influence of water body environmental pollution on aquatic organisms. Background technique: [0002] Polybrominated diphenyl ethers (PBDEs) are a kind of brominated flame retardants (BFRs), due to their high flame retardant efficiency, good thermal stability, small amount of addition, little impact on material properties, and low price , so as an additive flame retardant is widely used in electronics, electrical appliances, chemical transportation, building materials, textiles, petroleum, mining and other fields (Alaee M et al., 2003). PBDEs have strong lipophilicity and stable chemical properties, and can be bioaccumulated and amplified along the food chain. PBDEs enter the environment through evaporation and leakage during use and end-of-life. In addition, incineration of waste containing PBDEs is also the main way for PBDEs to enter the envir...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 童金苟谭新俞小牧程磊
Owner INST OF AQUATIC LIFE ACAD SINICA
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