Method for evaluating PBDEs xenoestrogen effect by using gobiocyprisrarus gene expression
A rare carp, gene expression technology, applied in the field of water environment evaluation, can solve the problems of longer time required for sperm and egg maturation, fewer sperm cells, etc.
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[0028] 1. Set up four fish ponds with the same environment, put polybrominated diphenyl ethers (polybrominated diphenyl ethers, PBDEs for short) water with a concentration of 1ppb in the first fish pond, which is called the first sample pond, and in the second fish pond Put water with PBDEs concentration of 10ppb in the pond, called the second sample pond, put water with PBDEs concentration of 100ppb in the third aquarium pond, called the third sample pond, and put it in the fourth aquarium pond The clear water without adding any medicine is called the sample pool of the control group;
[0029] 2. Put the rare squid fry that hatched for 2-3 days into four fish ponds respectively, and raise them under the same breeding conditions;
[0030] 3. After feeding for 3-5 months, fish out the same number of rare minnows from the four fish ponds, generally 30-200;
[0031] 4. All the rare goby fished in the first sample pool are called the first sample type, all the rare goby fished in...
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