Method for detecting gentamicin and/or micronomicin and special quantum dot fluorescence immunoassay kit thereof
A technology of gentamicin and quantum dots, which is applied in the field of detection of gentamicin and/or micronomycin and its special quantum dot fluorescence immunoassay kit, which can solve the problems of residual accumulation and slow excretion of animal products, It achieves the effects of good preservation and stability, simple sample pretreatment process and high sensitivity
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Embodiment 1
[0034] Example 1. Preparation and detection method of quantum dot fluorescence immunoassay kit
[0035] 1. Quantum Dot Fluorescence Immunoassay Kit includes:
[0036] (1) Obtained by dissolving the original coating in the coating buffer, wherein the concentration of the original coating in the original coating solution is 0.08 μg / mL; the original coating is the coupling of the gentamicin hapten and the carrier protein conjugates.
[0037] (2) Quantum dot-labeled goat anti-mouse anti-antibody working solution: obtained by diluting the quantum dot-labeled goat anti-mouse anti-antibody with diluent, and the dilution is 1:500;
[0038] The diluent is obtained by mixing bovine serum albumin and 50 mL of phosphate buffer; the concentration of the phosphate buffer is 0.02M, and the pH is 7.4.
[0039] Goat anti-mouse anti-antibody was purchased from Beijing Boaosen, catalog number is bs-0295G.
[0040] (3) Gentamicin standard solution: obtained by dissolving the standard in the di...
Embodiment 2
[0090] Example 2. Test kit sensitivity, accuracy and shelf life
[0091] 1. Kit sensitivity test
[0092] Perform 20 tests on the zero standard solution (that is, the diluent is phosphate buffer with pH 7.4 and 0.05M), and the average value of the determination results plus 3 times the standard deviation is used as the minimum detection limit of the kit.
[0093]Table 1 Statistical table of zero standard determination results μg / L
[0094]
[0095] It can be seen from Table 1 that the minimum detection limit of the kit is 1.0 μg / L.
[0096] Second, the standard precision test:
[0097] 10 kits were extracted from each batch of the three batches of kits (batch 01, batch 02, batch 03) described in Example 1, the luminescence intensity value of the 10 μg / L standard solution was measured, and the coefficient of variation was calculated. The detection method is the same as that described in Experiment 3 in Example 1.
[0098] The experiment was repeated three times, and the ...
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